A whole organism small molecule screen identifies novel regulators of pancreatic endocrine development.
Animals
Animals, Genetically Modified
COS Cells
Cell Differentiation
/ drug effects
Cell Transdifferentiation
/ drug effects
Cells, Cultured
Chlorocebus aethiops
Drug Evaluation, Preclinical
/ methods
Embryo, Nonmammalian
Gene Expression Regulation, Developmental
/ drug effects
Histone Deacetylase Inhibitors
/ isolation & purification
Homeodomain Proteins
/ genetics
Humans
Insulin-Secreting Cells
/ cytology
Islets of Langerhans
/ drug effects
Mice
Mice, Inbred C57BL
Models, Animal
Organogenesis
/ drug effects
Small Molecule Libraries
/ analysis
Trans-Activators
/ genetics
Valproic Acid
/ isolation & purification
Zebrafish
/ embryology
Pdx1
Small molecule screen
Transdifferentiation
α-Cells
β-Cells
Journal
Development (Cambridge, England)
ISSN: 1477-9129
Titre abrégé: Development
Pays: England
ID NLM: 8701744
Informations de publication
Date de publication:
24 07 2019
24 07 2019
Historique:
received:
08
10
2018
accepted:
08
05
2019
pubmed:
31
5
2019
medline:
7
7
2020
entrez:
31
5
2019
Statut:
epublish
Résumé
An early step in pancreas development is marked by the expression of the transcription factor Pdx1 within the pancreatic endoderm, where it is required for the specification of all endocrine cell types. Subsequently, Pdx1 expression becomes restricted to the β-cell lineage, where it plays a central role in β-cell function. This pivotal role of Pdx1 at various stages of pancreas development makes it an attractive target to enhance pancreatic β-cell differentiation and increase β-cell function. In this study, we used a newly generated zebrafish reporter to screen over 8000 small molecules for modulators of
Identifiants
pubmed: 31142539
pii: dev.172569
doi: 10.1242/dev.172569
pmc: PMC6679364
pii:
doi:
Substances chimiques
Histone Deacetylase Inhibitors
0
Homeodomain Proteins
0
Small Molecule Libraries
0
Trans-Activators
0
pancreatic and duodenal homeobox 1 protein
0
Valproic Acid
614OI1Z5WI
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIDDK NIH HHS
ID : U01 DK105541
Pays : United States
Informations de copyright
© 2019. Published by The Company of Biologists Ltd.
Déclaration de conflit d'intérêts
Competing interestsThe authors declare no competing or financial interests.
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