Structure and immunogenicity of a stabilized HIV-1 envelope trimer based on a group-M consensus sequence.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
29 05 2019
29 05 2019
Historique:
received:
09
10
2018
accepted:
26
04
2019
entrez:
31
5
2019
pubmed:
31
5
2019
medline:
18
6
2019
Statut:
epublish
Résumé
Stabilized HIV-1 envelope glycoproteins (Env) that resemble the native Env are utilized in vaccination strategies aimed at inducing broadly neutralizing antibodies (bNAbs). To limit the exposure of rare isolate-specific antigenic residues/determinants we generated a SOSIP trimer based on a consensus sequence of all HIV-1 group M isolates (ConM). The ConM trimer displays the epitopes of most known bNAbs and several germline bNAb precursors. The crystal structure of the ConM trimer at 3.9 Å resolution resembles that of the native Env trimer and its antigenic surface displays few rare residues. The ConM trimer elicits strong NAb responses against the autologous virus in rabbits and macaques that are significantly enhanced when it is presented on ferritin nanoparticles. The dominant NAb specificity is directed against an epitope at or close to the trimer apex. Immunogens based on consensus sequences might have utility in engineering vaccines against HIV-1 and other viruses.
Identifiants
pubmed: 31142746
doi: 10.1038/s41467-019-10262-5
pii: 10.1038/s41467-019-10262-5
pmc: PMC6541627
doi:
Substances chimiques
AIDS Vaccines
0
Antibodies, Neutralizing
0
Epitopes
0
HIV Antibodies
0
env Gene Products, Human Immunodeficiency Virus
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2355Subventions
Organisme : NIAID NIH HHS
ID : P01 AI110657
Pays : United States
Organisme : NIAID NIH HHS
ID : UM1 AI100663
Pays : United States
Références
PLoS Pathog. 2013 Jan;9(1):e1003106
pubmed: 23300456
PLoS Pathog. 2013 Sep;9(9):e1003618
pubmed: 24068931
Antiviral Res. 2018 Dec;160:25-37
pubmed: 30217650
J Virol. 2015 Sep;89(17):8932-44
pubmed: 26085151
J Immunol. 2012 Nov 15;189(10):4816-24
pubmed: 23066156
Immunity. 2015 Dec 15;43(6):1053-63
pubmed: 26682982
J Exp Med. 2017 Sep 4;214(9):2573-2590
pubmed: 28847869
Science. 2002 Jun 28;296(5577):2354-60
pubmed: 12089434
Retrovirology. 2018 Sep 12;15(1):63
pubmed: 30208933
Science. 2013 May 10;340(6133):711-6
pubmed: 23539181
Proc Natl Acad Sci U S A. 2015 Sep 22;112(38):11947-52
pubmed: 26372963
J Virol. 2013 Sep;87(17):9873-85
pubmed: 23824824
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):355-67
pubmed: 21460454
Cell Rep. 2018 Oct 23;25(4):893-908.e7
pubmed: 30355496
PLoS Pathog. 2015 Mar 25;11(3):e1004767
pubmed: 25807248
Virology. 2008 Mar 1;372(1):187-200
pubmed: 18031785
J Biol Chem. 2015 Mar 20;290(12):7436-42
pubmed: 25635058
Nature. 2014 Nov 6;515(7525):138-42
pubmed: 25186731
Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):12-21
pubmed: 20057044
Virol J. 2013 Apr 02;10:102
pubmed: 23548077
Acta Crystallogr D Struct Biol. 2016 Oct 1;72(Pt 10):1149-1161
pubmed: 27710936
Biochem Biophys Res Commun. 2009 Dec 18;390(3):404-9
pubmed: 19748484
Science. 2016 Jul 22;353(6297):389-94
pubmed: 27463675
Vaccine. 2014 Jan 9;32(3):327-37
pubmed: 24295808
J Virol. 2002 Mar;76(6):2606-16
pubmed: 11861826
Immunol Rev. 2017 Jan;275(1):230-244
pubmed: 28133800
Cell. 2014 Sep 25;159(1):69-79
pubmed: 25259921
J Virol. 2002 Sep;76(17):8875-89
pubmed: 12163607
Virology. 1991 Dec;185(2):661-72
pubmed: 1683726
Nature. 2014 Oct 23;514(7523):455-61
pubmed: 25296255
Science. 2013 Dec 20;342(6165):1484-90
pubmed: 24179160
Nat Commun. 2018 Sep 12;9(1):3693
pubmed: 30209313
Immunity. 2017 May 16;46(5):792-803.e3
pubmed: 28514686
Cell Rep. 2018 Apr 10;23(2):584-595
pubmed: 29642014
Cell Rep. 2017 Aug 22;20(8):1805-1817
pubmed: 28834745
Immunol Rev. 2017 Jan;275(1):161-182
pubmed: 28133806
Proc Natl Acad Sci U S A. 2008 Sep 9;105(36):13538-43
pubmed: 18765801
Methods Mol Biol. 2009;485:395-405
pubmed: 19020839
Cell Rep. 2018 Sep 18;24(12):3324-3338.e5
pubmed: 30232012
Nat Commun. 2018 May 16;9(1):1956
pubmed: 29769533
Retrovirology. 2014 Dec 20;11:125
pubmed: 25527085
J Virol. 2010 Feb;84(3):1439-52
pubmed: 19939925
Acta Crystallogr D Biol Crystallogr. 2001 Feb;57(Pt 2):254-9
pubmed: 11173472
Cell. 2016 May 5;165(4):813-26
pubmed: 27114034
Science. 2015 Jul 10;349(6244):aac4223
pubmed: 26089353
J Virol. 2000 Jan;74(2):627-43
pubmed: 10623724
J Virol. 2015 Oct;89(20):10383-98
pubmed: 26246566
Science. 2016 Mar 4;351(6277):1043-8
pubmed: 26941313
PLoS Pathog. 2018 Feb 23;14(2):e1006913
pubmed: 29474444
Expert Rev Vaccines. 2016;15(3):349-65
pubmed: 26654478
Science. 2013 Dec 20;342(6165):1477-83
pubmed: 24179159
Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32
pubmed: 15572765
J Virol. 2018 Jun 13;92(13):
pubmed: 29669838
J Virol. 2013 Apr;87(8):4185-201
pubmed: 23365441
J Virol. 2015 Dec 30;90(6):2806-17
pubmed: 26719252
Acta Crystallogr D Biol Crystallogr. 2007 Jan;63(Pt 1):32-41
pubmed: 17164524
Immunity. 2016 Sep 20;45(3):483-496
pubmed: 27617678
ACS Infect Dis. 2018 May 11;4(5):788-796
pubmed: 29451984
J Virol. 2018 Mar 28;92(8):
pubmed: 29367243
J Virol. 2015 Mar;89(6):3380-95
pubmed: 25589637
Science. 2017 Oct 13;358(6360):
pubmed: 28935768
Cell Rep. 2018 Aug 21;24(8):1958-1966.e5
pubmed: 30134158
Trends Immunol. 2016 Mar;37(3):221-232
pubmed: 26869204
Cell Rep. 2015 Jun 16;11(10):1604-13
pubmed: 26051934
J Virol. 2016 Nov 14;90(23):10587-10599
pubmed: 27654295
PLoS Pathog. 2016 Sep 14;12(9):e1005864
pubmed: 27627672
Nat Microbiol. 2016 Nov 14;2:16199
pubmed: 27841852
Virology. 2015 Dec;486:116-20
pubmed: 26433050
Cell Rep. 2016 Aug 30;16(9):2327-38
pubmed: 27545891
J Virol. 2015 Jun;89(12):6462-80
pubmed: 25855741
Nature. 2013 Jul 4;499(7456):102-6
pubmed: 23698367
Nat Rev Immunol. 2010 Nov;10(11):787-96
pubmed: 20948547
Cell. 2015 Dec 17;163(7):1702-15
pubmed: 26687358
Cell Rep. 2016 Mar 22;14(11):2695-706
pubmed: 26972002
Nat Commun. 2015 Sep 25;6:8167
pubmed: 26404402
Retrovirology. 2015 Sep 26;12:82
pubmed: 26410741
J Virol. 2014 Mar;88(5):2645-57
pubmed: 24352455
Immunol Rev. 2017 Jan;275(1):21-32
pubmed: 28133813
Virology. 2006 Sep 30;353(2):268-82
pubmed: 17039602
Nat Commun. 2016 Jun 28;7:12041
pubmed: 27349934