Detection of proteoglycan loss from articular cartilage using Brillouin microscopy, with applications to osteoarthritis.
Journal
Biomedical optics express
ISSN: 2156-7085
Titre abrégé: Biomed Opt Express
Pays: United States
ID NLM: 101540630
Informations de publication
Date de publication:
01 May 2019
01 May 2019
Historique:
received:
30
11
2018
revised:
21
02
2019
accepted:
22
02
2019
entrez:
31
5
2019
pubmed:
31
5
2019
medline:
31
5
2019
Statut:
epublish
Résumé
The degeneration of articular cartilage (AC) occurs in osteoarthritis (OA), which is a leading cause of pain and disability in middle-aged and older people. The early disease-related changes in cartilage extra-cellular matrix (ECM) start with depletion of proteoglycan (PG), leading to an increase in tissue hydration and permeability. These early compositional changes are small (<10%) and hence difficult to register with conventional non-invasive imaging technologies (magnetic resonance and ultrasound imaging). Here we apply Brillouin microscopy for detecting changes in the mechanical properties and composition of porcine AC. OA-like degradation is mimicked by enzymatic tissue digestion, and we compare Brillouin microscopy measurements against histological staining of PG depletion over varying digestion times and enzyme concentrations. The non-destructive nature of Brillouin imaging technology opens new avenues for creating minimally invasive arthroscopic devices for OA diagnostics and therapeutic monitoring.
Identifiants
pubmed: 31143498
doi: 10.1364/BOE.10.002457
pii: 353293
pmc: PMC6524605
doi:
Types de publication
Journal Article
Langues
eng
Pagination
2457-2466Déclaration de conflit d'intérêts
The author have no conflict of interest.
Références
Nat Nanotechnol. 2009 Mar;4(3):186-92
pubmed: 19265849
Nat Photonics. 2007 Dec 9;2:39-43
pubmed: 19812712
Connect Tissue Res. 2010;51(1):36-47
pubmed: 20067415
Foot (Edinb). 2009 Sep;19(3):171-6
pubmed: 20307471
Bone. 2012 Aug;51(2):278-88
pubmed: 22155587
J Microsc. 2012 Dec;248(3):281-91
pubmed: 23140377
HSS J. 2012 Feb;8(1):7-9
pubmed: 23372517
Quant Imaging Med Surg. 2013 Jun;3(3):162-74
pubmed: 23833729
J Bone Joint Surg Am. 2014 Jul 16;96(14):1145-1151
pubmed: 25031368
Osteoarthritis Cartilage. 2014 Oct;22(10):1419-28
pubmed: 25278053
J R Soc Interface. 2014 Dec 6;11(101):20140739
pubmed: 25297313
Nat Methods. 2015 Dec;12(12):1132-4
pubmed: 26436482
J R Soc Interface. 2015 Nov 6;12(112):
pubmed: 26559685
Faraday Discuss. 2016 Jun 23;187:415-28
pubmed: 27051893
Sci Rep. 2016 Nov 15;6:37217
pubmed: 27845411
Nat Methods. 2018 Aug;15(8):561-562
pubmed: 30065366
J Mol Biol. 1979 Nov 25;135(1):39-51
pubmed: 529291
Nature. 1977 May 19;267(5608):285-7
pubmed: 865624
Instr Course Lect. 1998;47:487-504
pubmed: 9571450