Spectral image scanning microscopy.
Journal
Biomedical optics express
ISSN: 2156-7085
Titre abrégé: Biomed Opt Express
Pays: United States
ID NLM: 101540630
Informations de publication
Date de publication:
01 May 2019
01 May 2019
Historique:
received:
08
02
2019
revised:
12
04
2019
accepted:
15
04
2019
entrez:
31
5
2019
pubmed:
31
5
2019
medline:
31
5
2019
Statut:
epublish
Résumé
For decades, the confocal microscope has represented one of the dominant imaging systems in biomedical imaging at sub-cellular lengthscales. Recently, however, it has increasingly been replaced by a related, but more powerful successor technique termed image scanning microscopy (ISM). In this article, we present ISM capable of measuring spectroscopic information such as that contained in fluorescence or Raman images. Compared to established confocal spectroscopic imaging systems, our implementation offers similar spectral resolution, but higher spatial resolution and detection efficiency. Color sensitivity is achieved by a grating placed in the detection path in conjunction with a camera collecting both spatial and spectral information. The multidimensional data is processed using multi-view maximum likelihood image reconstruction. Our findings are supported by numerical simulations and experiments on micro beads and double-stained HeLa cells.
Identifiants
pubmed: 31143501
doi: 10.1364/BOE.10.002513
pii: 359823
pmc: PMC6524570
doi:
Types de publication
Journal Article
Langues
eng
Pagination
2513-2527Déclaration de conflit d'intérêts
The authors declare that there are no conflicts of interest related to this article.
Références
Biophys J. 2001 Feb;80(2):986-93
pubmed: 11159465
Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):5788-92
pubmed: 11959908
FEBS Lett. 2003 Jul 3;546(1):87-92
pubmed: 12829241
Cytometry A. 2004 Nov;62(1):8-34
pubmed: 15468110
Opt Lett. 2006 Jan 15;31(2):181-3
pubmed: 16441023
Opt Express. 2002 Jan 14;10(1):98-103
pubmed: 19424335
Opt Lett. 1991 Aug 15;16(16):1277-9
pubmed: 19776944
Opt Express. 2009 Oct 26;17(22):19644-55
pubmed: 19997184
Phys Rev Lett. 2010 May 14;104(19):198101
pubmed: 20867000
Appl Opt. 1995 Apr 10;34(11):1859-66
pubmed: 21037731
Nat Methods. 2012 May 13;9(7):749-54
pubmed: 22581372
J Microsc. 2012 Aug;247(2):147-60
pubmed: 22612666
Proc Natl Acad Sci U S A. 2012 Nov 20;109(47):19087-92
pubmed: 23129640
Opt Express. 2013 Mar 11;21(5):6584-600
pubmed: 23482230
Opt Lett. 2013 May 1;38(9):1521-3
pubmed: 23632538
Opt Lett. 2013 Aug 1;38(15):2889-92
pubmed: 23903171
Nat Methods. 2013 Nov;10(11):1122-6
pubmed: 24097271
Biomed Opt Express. 2013 Oct 25;4(11):2644-56
pubmed: 24298422
Proc Natl Acad Sci U S A. 2013 Dec 24;110(52):21000-5
pubmed: 24324140
Chemphyschem. 2014 Mar 17;15(4):794-800
pubmed: 24436314
Opt Express. 2014 Feb 24;22(4):4029-37
pubmed: 24663724
Opt Lett. 2014 Jun 1;39(11):3352-5
pubmed: 24876051
Optica. 2014 Sep 20;1(3):181-191
pubmed: 25485291
Opt Express. 2015 Jun 1;23(11):15003-11
pubmed: 26072856
J Microsc. 2016 Jun;262(3):306-15
pubmed: 26694787
Opt Express. 2016 Jul 11;24(14):15456-67
pubmed: 27410820
Opt Lett. 2016 Aug 15;41(16):3825-8
pubmed: 27519099
Nat Methods. 2017 Nov;14(11):1087-1089
pubmed: 29039418
Nat Methods. 2019 Feb;16(2):175-178
pubmed: 30643212