Early ovine preantral follicles have a potential to grow until antral stage in two-step culture system in the presence of aqueous extract of Justicia insularis.


Journal

Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
Titre abrégé: Reprod Domest Anim
Pays: Germany
ID NLM: 9015668

Informations de publication

Date de publication:
Aug 2019
Historique:
received: 19 03 2019
accepted: 25 05 2019
pubmed: 31 5 2019
medline: 1 2 2020
entrez: 31 5 2019
Statut: ppublish

Résumé

The objective of this study was to determine whether preantral follicles cultured in vitro for 7 days within ovine ovarian cortical strips could be isolated at the secondary follicles (SF) and grown until antral stage during an additional 6 days period of in vitro culture in the presence of aqueous extract of Justicia insularis. Fresh ovarian fragments from 16 adult sheep were fixed for histological analysis (Control 1) or in vitro cultured individually in α-MEM

Identifiants

pubmed: 31145489
doi: 10.1111/rda.13477
doi:

Substances chimiques

Culture Media 0
Plant Extracts 0
Trehalose B8WCK70T7I

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1121-1130

Subventions

Organisme : National Council for Scientific and Technological Development
ID : 308071/2016-6

Informations de copyright

© 2019 Blackwell Verlag GmbH.

Auteurs

Gildas Tetaping Mbemya (GT)

Laboratory of Manipulation of Oocyte and Ovarian Preantral Follicles (LAMOFOPA), Faculty of Veterinary (FAVET), State University of Ceará, Fortaleza, Brazil.

Naiza Arcângela Ribeiro de Sá (NAR)

Laboratory of Manipulation of Oocyte and Ovarian Preantral Follicles (LAMOFOPA), Faculty of Veterinary (FAVET), State University of Ceará, Fortaleza, Brazil.

Denise Damasceno Guerreiro (DD)

Laboratory of Manipulation of Oocyte and Ovarian Preantral Follicles (LAMOFOPA), Faculty of Veterinary (FAVET), State University of Ceará, Fortaleza, Brazil.

Francisca Geovania Canafístula de Sousa (FGC)

Laboratory of Manipulation of Oocyte and Ovarian Preantral Follicles (LAMOFOPA), Faculty of Veterinary (FAVET), State University of Ceará, Fortaleza, Brazil.

Sylvain Njina Nguedia (SN)

Laboratory of Manipulation of Oocyte and Ovarian Preantral Follicles (LAMOFOPA), Faculty of Veterinary (FAVET), State University of Ceará, Fortaleza, Brazil.

Benner Geraldo Alves (BG)

Laboratory of Biology of Reproduction, Federal University of Uberlândia, Uberlândia, Brazil.

Francielli Weber Santos (FW)

Laboratory of Reproduction Biotechnology (Biotech), State of University of Pampa, Uruguaiana, Brazil.

Otília Deusdênia Loiola Pessoa (ODL)

Laboratory of Phytochemical Analysis of Medicinal Plants (LAFIPLAN), Federal University of Ceará, Fortaleza, Brazil.

Pierre Comizzoli (P)

Center for Species Survival, Smithsonian Conservation Biology Institute, Front Royal, Virginia, USA.

José Ricardo Figueiredo (JR)

Laboratory of Manipulation of Oocyte and Ovarian Preantral Follicles (LAMOFOPA), Faculty of Veterinary (FAVET), State University of Ceará, Fortaleza, Brazil.

Ana Paula Ribeiro Rodrigues (APR)

Laboratory of Manipulation of Oocyte and Ovarian Preantral Follicles (LAMOFOPA), Faculty of Veterinary (FAVET), State University of Ceará, Fortaleza, Brazil.

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Classifications MeSH