Nephroprotective activity of Combretum micranthum G. Don in cisplatin induced nephrotoxicity in rats: In-vitro, in-vivo and in-silico experiments.
Animals
Biomarkers
/ blood
Body Weight
/ drug effects
Cisplatin
/ adverse effects
Combretum
/ chemistry
Computer Simulation
HEK293 Cells
Humans
Kidney
/ drug effects
Male
Molecular Docking Simulation
Molecular Dynamics Simulation
Oxidative Stress
/ drug effects
Plant Extracts
/ pharmacology
Protective Agents
/ pharmacology
Rats, Wistar
HEK-293
Kidney
Molecular docking
Molecular dynamic studies
Nephrotoxicity
Oxidative stress
Journal
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
ISSN: 1950-6007
Titre abrégé: Biomed Pharmacother
Pays: France
ID NLM: 8213295
Informations de publication
Date de publication:
Aug 2019
Aug 2019
Historique:
received:
04
04
2019
revised:
02
05
2019
accepted:
08
05
2019
pubmed:
31
5
2019
medline:
7
1
2020
entrez:
31
5
2019
Statut:
ppublish
Résumé
Nephrotoxicity is known to be a major complication during cisplatin chemotherapy in cancer patients. In the present study, the protective effect of a hydroalcoholic extract of Combretum micranthum (CM) against cisplatin (CP)-induced renal damage was evaluated using in-vitro human embryonic kidney (HEK)-293 cells and in-vivo experiments. Further, in-silico molecular docking and dynamic experiments were carried out with bioactive compounds of the title plant against nuclear factor kappa B (NF-κB) and soluble epoxide hydrolase (sEH). Incubation of HEK-293 cells with cisplatin resulted in a significant increase in cell death with changes in normal cellular morphology. Co-treatment of HEK-293 cells with CP and CM extract at varying concentrations resulted in significant enhancement of cell growth compared to CP treatment indicating the cytoprotective activity of CM with an EC
Identifiants
pubmed: 31146106
pii: S0753-3322(19)31512-4
doi: 10.1016/j.biopha.2019.108961
pii:
doi:
Substances chimiques
Biomarkers
0
Plant Extracts
0
Protective Agents
0
Cisplatin
Q20Q21Q62J
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
108961Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier Masson SAS.. All rights reserved.