A tessellation-based colocalization analysis approach for single-molecule localization microscopy.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
30 05 2019
30 05 2019
Historique:
received:
16
05
2018
accepted:
12
04
2019
entrez:
1
6
2019
pubmed:
31
5
2019
medline:
31
5
2019
Statut:
epublish
Résumé
Multicolor single-molecule localization microscopy (λSMLM) is a powerful technique to reveal the relative nanoscale organization and potential colocalization between different molecular species. While several standard analysis methods exist for pixel-based images, λSMLM still lacks such a standard. Moreover, existing methods only work on 2D data and are usually sensitive to the relative molecular organization, a very important parameter to consider in quantitative SMLM. Here, we present an efficient, parameter-free colocalization analysis method for 2D and 3D λSMLM using tessellation analysis. We demonstrate that our method allows for the efficient computation of several popular colocalization estimators directly from molecular coordinates and illustrate its capability to analyze multicolor SMLM data in a robust and efficient manner.
Identifiants
pubmed: 31147535
doi: 10.1038/s41467-019-10007-4
pii: 10.1038/s41467-019-10007-4
pmc: PMC6542817
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
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