Analysis of Proteasome-Generated Antigenic Peptides by Mass Spectrometry.
Antigen processing
Antigenic peptides
Epitope
Fragment generation
HPLC
Mass spectrometry
Polypeptides
Proteasome
Substrate degradation
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
1
6
2019
pubmed:
31
5
2019
medline:
23
1
2020
Statut:
ppublish
Résumé
Mass spectrometry (MS) is today one of the most important analytical techniques in biosciences. The development of electro spray ionization (ESI) as a gentle method, in which molecules are not destroyed, has revolutionized the analytic of peptides. MS is an ideal technique for detection and analysis of peptides generated by purified 20S proteasomes in in vitro experiments. This approach also provides a convenient and sensitive way to monitor the different processing characteristics of proteasome isoforms. The combination of high performance liquid chromatography (HPLC) with ESI-MS allows for the analysis of complex samples with separation in their specific constituents by LC and their subsequent detection by MS.
Identifiants
pubmed: 31147929
doi: 10.1007/978-1-4939-9450-2_2
doi:
Substances chimiques
Antigens
0
Peptides
0
Proteasome Endopeptidase Complex
EC 3.4.25.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM