NP and NS1 proteins of H5N1 virus significantly upregulated IFITM1, IFITM2, and IFITM3 in A549 cells.
A549 Cells
Antigens, Differentiation
Carrier Proteins
HEK293 Cells
Humans
Influenza A Virus, H5N1 Subtype
/ pathogenicity
Membrane Proteins
/ genetics
Nucleocapsid Proteins
RNA-Binding Proteins
/ genetics
Real-Time Polymerase Chain Reaction
Viral Core Proteins
Viral Nonstructural Proteins
/ genetics
Viral Proteins
Virus Internalization
H5N1
IFITMs
Influenza Virus
NP protein
NS1 protein
Journal
African health sciences
ISSN: 1729-0503
Titre abrégé: Afr Health Sci
Pays: Uganda
ID NLM: 101149451
Informations de publication
Date de publication:
Mar 2019
Mar 2019
Historique:
entrez:
1
6
2019
pubmed:
1
6
2019
medline:
19
11
2019
Statut:
ppublish
Résumé
Avian influence virus H5N1 causes serious public health concern with significant morbidity and mortality from poultry to humans. Interferon-induced transmembrane (IFITM) proteins usually protect cells from many virus infections by viral entry and replication. The purpose of this study was to investigate whether H5N1 viral proteins involved in regulation IFITM1, IFITM2, and IFITM3 following H5N1 infection. NS1, M1, NP, PB2, HA and NA genes of H5N1 virus were generated by PCR and cloned into pcDNA3.1/myc-His (+) A vector for genes over-expression experiments. Gene expression levels was performed using Real-time PCR. Research displayed that NS1, M1, NP, and PB2 proteins of H5N1 virus increased IFITM1, IFITM2, and IFITM3 expression in A549 cells, only IFITM1 was upregulated by M1 in HEK293T cells. However, our study did not find that HA and NA of H5N1 virus affected IFITM genes family or interferon genes expression. Taken together, our data suggested that IFITM1, IFITM2, and IFITM3 might be directly upregulated via NS1, M1, NP, and PB2 proteins during H5N1 avian influenza virus infection. This study provided new insights into the influence of NS1 and NP proteins on regulation of IFITM1, IFITM2, and IFITM3 expression following H5N1 infection.
Sections du résumé
BACKGROUND
BACKGROUND
Avian influence virus H5N1 causes serious public health concern with significant morbidity and mortality from poultry to humans. Interferon-induced transmembrane (IFITM) proteins usually protect cells from many virus infections by viral entry and replication.
OBJECTIVES
OBJECTIVE
The purpose of this study was to investigate whether H5N1 viral proteins involved in regulation IFITM1, IFITM2, and IFITM3 following H5N1 infection.
METHODS
METHODS
NS1, M1, NP, PB2, HA and NA genes of H5N1 virus were generated by PCR and cloned into pcDNA3.1/myc-His (+) A vector for genes over-expression experiments. Gene expression levels was performed using Real-time PCR.
RESULTS
RESULTS
Research displayed that NS1, M1, NP, and PB2 proteins of H5N1 virus increased IFITM1, IFITM2, and IFITM3 expression in A549 cells, only IFITM1 was upregulated by M1 in HEK293T cells. However, our study did not find that HA and NA of H5N1 virus affected IFITM genes family or interferon genes expression.
CONCLUSION
CONCLUSIONS
Taken together, our data suggested that IFITM1, IFITM2, and IFITM3 might be directly upregulated via NS1, M1, NP, and PB2 proteins during H5N1 avian influenza virus infection. This study provided new insights into the influence of NS1 and NP proteins on regulation of IFITM1, IFITM2, and IFITM3 expression following H5N1 infection.
Identifiants
pubmed: 31148967
doi: 10.4314/ahs.v19i1.13
pii: jAFHS.v19.i1.pg1402
pmc: PMC6531959
doi:
Substances chimiques
Antigens, Differentiation
0
Carrier Proteins
0
IFITM2 protein, human
0
IFITM3 protein, human
0
INS1 protein, influenza virus
0
Membrane Proteins
0
NP protein, Influenza A virus
0
Nucleocapsid Proteins
0
RNA-Binding Proteins
0
Viral Core Proteins
0
Viral Nonstructural Proteins
0
Viral Proteins
0
leu-13 antigen
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
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