Smartphone operable centrifugal system (SOCS) for on-site DNA extraction from foodborne bacterial pathogen.
Journal
Biomicrofluidics
ISSN: 1932-1058
Titre abrégé: Biomicrofluidics
Pays: United States
ID NLM: 101293825
Informations de publication
Date de publication:
May 2019
May 2019
Historique:
received:
25
02
2019
accepted:
06
05
2019
entrez:
1
6
2019
pubmed:
1
6
2019
medline:
1
6
2019
Statut:
epublish
Résumé
The on-site recovery of nucleic acid from foodborne bacteria is in high demand to further understand on-site molecular diagnosis, which is especially applicable in developing countries. Here, we first proposed a smartphone operable centrifugal system (SOCS) for nucleic acid extraction with the assistance of a low power consumable motor and hydrogel beads. The SOCS consists of a centrifugal motor, 3D-printed cartridge, a nucleic acid collection column, and a smartphone. The SOCS shows excellent DNA extraction performance within 6 min, and it can operate more than 100 times using a smartphone. The purified effluent DNA was accumulated in the nucleic acid collection column. The performance of the SOCS was confirmed by amplifying the recovered DNA from
Identifiants
pubmed: 31149321
doi: 10.1063/1.5093752
pii: 1.5093752
pii: 011903BMF
pmc: PMC6531255
doi:
Types de publication
Journal Article
Langues
eng
Pagination
034111Références
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