Profilin2 is required for filamentous actin formation induced by human parainfluenza virus type 2.
Filamentous actin
Human parainfluenza virus type 2
V protein
profilin2
Journal
Virology
ISSN: 1096-0341
Titre abrégé: Virology
Pays: United States
ID NLM: 0110674
Informations de publication
Date de publication:
07 2019
07 2019
Historique:
received:
14
02
2019
revised:
09
05
2019
accepted:
23
05
2019
pubmed:
1
6
2019
medline:
5
11
2019
entrez:
1
6
2019
Statut:
ppublish
Résumé
We previously reported that human parainfluenza virus type 2 (hPIV-2) promoted RhoA activation and subsequent filamentous actin (F-actin) formation. Actin-binding proteins, such as profilin and cofilin, are involved in the regulation of F-actin formation by RhoA signaling. In the present study, we identified profilin2 as a key molecule that is involved in hPIV-2-induced F-actin formation. Immunoprecipitation assays demonstrated that hPIV-2 V protein binds to profilin2 but not to profilin1. Mutation of Trp residues within C-terminal region of V protein abolished the binding capacity to profilin2. Depletion of profilin2 resulted in the inhibition of hPIV-2-induced F-actin formation and the suppression of hPIV-2 growth. Overexpression of wild type V but not Trp-mutated V protein reduced the quantity of actin co-immunoprecipitated with profilin2. Taken together, these results suggest that hPIV-2 V protein promotes F-actin formation by affecting actin-profilin2 interaction through its binding to profilin2.
Identifiants
pubmed: 31150988
pii: S0042-6822(19)30133-3
doi: 10.1016/j.virol.2019.05.013
pii:
doi:
Substances chimiques
Actins
0
Profilins
0
V protein, human parainfluenza virus type 1
0
Viral Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
108-114Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.