Cercospora sp. as a source of anti-aging polyketides targeting 26S proteasome and scale-up production in submerged bioreactor.
Antioxidant activity
Bioreactor
Cercospora
Filamentous fungi
Polyketides
Proteasome
Journal
Journal of biotechnology
ISSN: 1873-4863
Titre abrégé: J Biotechnol
Pays: Netherlands
ID NLM: 8411927
Informations de publication
Date de publication:
10 Aug 2019
10 Aug 2019
Historique:
received:
31
01
2019
revised:
14
05
2019
accepted:
27
05
2019
pubmed:
4
6
2019
medline:
18
12
2019
entrez:
2
6
2019
Statut:
ppublish
Résumé
From a large screening of microbial extracts for the discovery of proteasome modulating natural products, the fungal strain Cercospora sp. (CF-223709) was selected as the most promising for further investigation. Different liquid cultures of the strain were initially screened for their anti-oxidant activity (DPPH, ABTS) and for their cytotoxicity against the A2058, HepG2 and CCD25sk cell lines. A detailed chemical analysis and evaluation of the capacity to activate 26S-proteasome was followed for the most active extract. Three main polyketides were isolated and characterized by extensive analysis of NMR and HRMS spectra data as penialidine F (1), fulvic acid (2), and SB238569 (3). Fulvic acid showed the most significant anti-oxidant activity. Its IC
Identifiants
pubmed: 31152756
pii: S0168-1656(19)30179-8
doi: 10.1016/j.jbiotec.2019.05.015
pii:
doi:
Substances chimiques
Antioxidants
0
Biological Products
0
Polyketides
0
Proteasome Endopeptidase Complex
EC 3.4.25.1
ATP dependent 26S protease
EC 3.4.99.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
88-96Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.