Microwave ablation zones are larger than they macroscopically appear - Reevaluation based on NADH vitality staining ex vivo.

Ablation Techniques / methods Animals Catheter Ablation / methods Disease Models, Animal NAD / metabolism Research Design Staining and Labeling Swine
Liver NADH-diaphorase animal model ex vivo microwave ablation (MWA) viability staining

Journal

Clinical hemorheology and microcirculation
ISSN: 1875-8622
Titre abrégé: Clin Hemorheol Microcirc
Pays: Netherlands
ID NLM: 9709206

Informations de publication

Date de publication:
2019
Historique:
pubmed: 4 6 2019
medline: 4 3 2020
entrez: 4 6 2019
Statut: ppublish

Résumé

Animal liver is established as an ex vivo model for studies on hepatic microwave ablation (MWA). Macroscopically visible color changes in the ablation zone are used to assess cell destruction and confirm successful ablation ex vivo. Macroscopy and histology of MWA zones regarding cell viability in ex vivo porcine livers were compared in this study. Six MWA were performed in porcine livers post mortem. A 14-G antenna and microwave generator (928 MHz; 9.0 kJ) were used. MWA were cut at the maximum cross section in vertical alignment to the antenna. NADH-diaphorase staining determined cell vitality. Macroscopic and microscopic ablation zones were statistically analyzed. Histology showed two distinct ablation zones: central white zone (WZH) with no cell viability and peripheral red zone (RZH) with partial cell viability. However, the macroscopically visible WZM was significantly smaller than the microscopic WZH with an area difference of 43.1% (p < 0.05) and a radius difference of 21.2% (1.6 mm; p < 0.05). Macroscopy and histology showed a very high correlation for the complete lesion area (WZH/M+RZH/M; r = 0.9; p = 0.001). The avital central zone is significantly larger as the macroscopically visible WZ which is commonly used to assess successful ablation in MWA ex vivo studies. Irreversible cell destruction can be underestimated in macroscopic evaluation.

Sections du résumé

BACKGROUND BACKGROUND
Animal liver is established as an ex vivo model for studies on hepatic microwave ablation (MWA). Macroscopically visible color changes in the ablation zone are used to assess cell destruction and confirm successful ablation ex vivo.
OBJECTIVE OBJECTIVE
Macroscopy and histology of MWA zones regarding cell viability in ex vivo porcine livers were compared in this study.
METHODS METHODS
Six MWA were performed in porcine livers post mortem. A 14-G antenna and microwave generator (928 MHz; 9.0 kJ) were used. MWA were cut at the maximum cross section in vertical alignment to the antenna. NADH-diaphorase staining determined cell vitality. Macroscopic and microscopic ablation zones were statistically analyzed.
RESULTS RESULTS
Histology showed two distinct ablation zones: central white zone (WZH) with no cell viability and peripheral red zone (RZH) with partial cell viability. However, the macroscopically visible WZM was significantly smaller than the microscopic WZH with an area difference of 43.1% (p < 0.05) and a radius difference of 21.2% (1.6 mm; p < 0.05). Macroscopy and histology showed a very high correlation for the complete lesion area (WZH/M+RZH/M; r = 0.9; p = 0.001).
CONCLUSIONS CONCLUSIONS
The avital central zone is significantly larger as the macroscopically visible WZ which is commonly used to assess successful ablation in MWA ex vivo studies. Irreversible cell destruction can be underestimated in macroscopic evaluation.

Identifiants

DOI: 10.3233/CH-190583 PMID: 31156148
pubmed: 31156148
pii: CH190583
doi: 10.3233/CH-190583
doi:

Substances chimiques

NAD 0U46U6E8UK

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

371-378

Auteurs

Beatrice Geyer (B)

Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Department of General, Visceral and Vascular Surgery - Campus Benjamin Franklin, Berlin, Germany.

Franz G M Poch (FGM)

Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Department of General, Visceral and Vascular Surgery - Campus Benjamin Franklin, Berlin, Germany.

Ole Gemeinhardt (O)

Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Department of Radiology - Campus Mitte, Berlin, Germany.

Christina A Neizert (CA)

Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Department of General, Visceral and Vascular Surgery - Campus Benjamin Franklin, Berlin, Germany.

Stefan M Niehues (SM)

Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Department of Radiology - Campus Benjamin Franklin, Berlin, Germany.

Janis L Vahldiek (JL)

Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Department of Radiology - Campus Benjamin Franklin, Berlin, Germany.

Robert Klopfleisch (R)

Institute of Veterinary Pathology, Freie Universität Berlin, Berlin, Germany.

Kai S Lehmann (KS)

Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Department of General, Visceral and Vascular Surgery - Campus Benjamin Franklin, Berlin, Germany.

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Classifications MeSH

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