Human Enriched Serum Following Hydrolysed Collagen Absorption Modulates Bone Cell Activity: from Bedside to Bench and Vice Versa.
3T3 Cells
Animals
Bone Density
Bone Marrow Cells
Bone and Bones
/ cytology
Cell Proliferation
Collagen
/ administration & dosage
Dietary Supplements
Female
Gene Expression Regulation
/ drug effects
Humans
Hydrolysis
Leukocytes, Mononuclear
/ drug effects
Mice
Mice, Inbred C3H
Osteoclasts
/ drug effects
Ovariectomy
RANK Ligand
/ genetics
RAW 264.7 Cells
Random Allocation
absorption
bone
collagen peptides
hydrolysed collagen
metabolites
nutrition
osteoporosis
Journal
Nutrients
ISSN: 2072-6643
Titre abrégé: Nutrients
Pays: Switzerland
ID NLM: 101521595
Informations de publication
Date de publication:
31 May 2019
31 May 2019
Historique:
received:
24
04
2019
revised:
27
05
2019
accepted:
28
05
2019
entrez:
5
6
2019
pubmed:
5
6
2019
medline:
7
1
2020
Statut:
epublish
Résumé
Collagen proteins are crucial components of the bone matrix. Since collagen-derived products are widely used in the food and supplement industry, one may raise the question whether collagen-enriched diets can provide benefits for the skeleton. In this study, we designed an innovative approach to investigate this question taking into account the metabolites that are formed by the digestive tract and appear in the circulation after ingestion of hydrolysed collagen. Blood samples collected in clinical and pre-clinical trials following ingestion and absorption of hydrolysed collagen were processed and applied on bone-related primary cell cultures. This original ex vivo methodology revealed that hydrolysed collagen-enriched serum had a direct impact on the behaviour of cells from both human and mouse origin that was not observed with controls (bovine serum albumin or hydrolysed casein-enriched serum). These ex vivo findings were fully in line with in vivo results obtained from a mouse model of post-menopausal osteoporosis. A significant reduction of bone loss was observed in mice supplemented with hydrolysed collagen compared to a control protein. Both the modulation of osteoblast and osteoclast activity observed upon incubation with human or mouse serum ex vivo and the attenuation of bone loss in vivo, clearly indicates that the benefits of hydrolysed collagen for osteoporosis prevention go beyond the effect of a simple protein supplementation.
Identifiants
pubmed: 31159319
pii: nu11061249
doi: 10.3390/nu11061249
pmc: PMC6627680
pii:
doi:
Substances chimiques
RANK Ligand
0
Collagen
9007-34-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Association Nationale de la Recherche et de la Technologie
ID : COLOS
Déclaration de conflit d'intérêts
Fabien Wauquier, Henri Granel, Audrey Daneault, Gael Rochefort, Jérome Guicheux, Adeline Blot, Nathalie Meunier and Yohann Wittrant have no conflict of interest to declare. Janne Prawitt and Véronique Fabien-Soulé work for Rousselot and provided the hydrolysed collagens.
Références
Semin Arthritis Rheum. 2000 Oct;30(2):87-99
pubmed: 11071580
J Biochem. 2001 Jan;129(1):133-8
pubmed: 11134967
J Bone Miner Metab. 2002;20(5):298-302
pubmed: 12203036
Int J Clin Pharmacol Res. 2002;22(3-4):101-10
pubmed: 12837047
Osteoporos Int. 2003 Sep;14 Suppl 5:S29-36
pubmed: 14504703
Osteoporos Int. 2006;17(3):319-36
pubmed: 16341622
Biosci Biotechnol Biochem. 2007 Nov;71(11):2680-7
pubmed: 17986775
Bone. 2008 Jun;42(6):1122-30
pubmed: 18378205
Maturitas. 2010 Mar;65(3):253-7
pubmed: 19892499
Bone. 2010 Mar;46(3):827-34
pubmed: 19895915
J Agric Food Chem. 2010 Jan 27;58(2):835-41
pubmed: 19957932
Int J Food Sci Nutr. 2010 Feb;61(1):52-60
pubmed: 19961355
Int Orthop. 2011 Mar;35(3):341-8
pubmed: 20401752
Osteoarthritis Cartilage. 2011 Jan;19(1):1-21
pubmed: 21035558
Osteoporos Int. 2012 Jan;23(1):193-9
pubmed: 21350895
Cold Spring Harb Perspect Biol. 2011 Jan 01;3(1):a004978
pubmed: 21421911
Osteoporos Int. 2012 Jul;23(7):1909-19
pubmed: 21927918
Biol Pharm Bull. 2012;35(3):422-7
pubmed: 22382331
Complement Ther Med. 2012 Jun;20(3):124-30
pubmed: 22500661
Osteoporos Int. 2013 Jan;24(1):139-50
pubmed: 22707061
Osteoporos Int. 2013 Mar;24(3):771-86
pubmed: 23152094
Dent Mater J. 2013;32(1):88-95
pubmed: 23370875
Dent Mater J. 2013;32(2):233-40
pubmed: 23538758
J Med Food. 2013 May;16(5):447-53
pubmed: 23631489
Molecules. 2013 Dec 12;18(12):15474-89
pubmed: 24352008
Cell Biol Int. 2014 May;38(5):655-62
pubmed: 24448827
Food Funct. 2014 Mar;5(3):573-8
pubmed: 24496382
Food Chem. 2014 Sep 15;159:328-32
pubmed: 24767063
J Sci Food Agric. 2015 Mar 15;95(4):702-7
pubmed: 24852756
PLoS One. 2014 Jun 13;9(6):e99920
pubmed: 24926875
J Med Food. 2015 Mar;18(3):324-31
pubmed: 25314004
Front Immunol. 2014 Oct 20;5:511
pubmed: 25368616
Eur J Nutr. 2015 Oct;54(7):1139-49
pubmed: 25370302
J Agric Food Chem. 2014 Dec 17;62(50):12096-102
pubmed: 25417748
Crit Rev Food Sci Nutr. 2017 Jun 13;57(9):1922-1937
pubmed: 25976422
Biomed Mater. 2015 Dec 14;10(6):065020
pubmed: 26657831
J Musculoskelet Neuronal Interact. 2016 Jun 01;16(2):161-7
pubmed: 27282460
Calcif Tissue Int. 2017 May;100(5):431-432
pubmed: 28154889
Am J Med. 2017 Jul;130(7):862.e1-862.e7
pubmed: 28359721
J Agric Food Chem. 2017 Nov 1;65(43):9514-9521
pubmed: 28988478
Br J Sports Med. 2018 Feb;52(3):167-175
pubmed: 29018060
BMC Vet Res. 2017 Dec 20;13(1):395
pubmed: 29262825
Nutrients. 2018 Jan 16;10(1):null
pubmed: 29337906
Bone. 2018 Oct;115:15-24
pubmed: 29499416
Nutrients. 2018 Oct 04;10(10):null
pubmed: 30287779
Cas Lek Cesk. 1996 Jan 31;135(3):74-8
pubmed: 8625373