LINE-1 methylation in cleft lip tissues: Influence of infant MTHFR c.677C>T genotype.
DNA methylation
LINE-1
MTHFR c.677C>T
non-syndromic cleft lip with or without cleft palate
Journal
Oral diseases
ISSN: 1601-0825
Titre abrégé: Oral Dis
Pays: Denmark
ID NLM: 9508565
Informations de publication
Date de publication:
Sep 2019
Sep 2019
Historique:
received:
07
01
2019
revised:
06
05
2019
accepted:
24
05
2019
pubmed:
5
6
2019
medline:
31
12
2019
entrez:
5
6
2019
Statut:
ppublish
Résumé
To investigate the influence of MTHFR c.677C>T genotype on LINE-1 methylation in lateral and medial tissues from cleft lip (CL). Forty-five consecutive non-syndromic cleft lip with or without cleft palate (nsCL/P) cases were included in the study. Genomic DNA was extracted from tissues at both sides of cleft lip, and LINE-1 methylation was detected by bisulfite conversion and pyrosequencing. MTHFR c.677C>T genotyping was carried out using the TaqMan genotyping assay. LINE-1 methylation level was significantly higher on medial side of cleft lip compared with lateral side (p = 0.001). This difference was not significantly influenced by the case's sex or cleft type. However, MTHFR c.677C>T genotyping revealed that the difference in LINE-1 methylation across cleft lip was restricted to carriers of C allele of MTHFR c.677C>T and was not apparent in TT homozygous cases (p = 0.027). This integrated analysis supports the previous finding of differences in DNA methylation across the two sides of cleft lip and further suggests a possible role of MTHFR c.677C>T genotype in establishing this difference.
Substances chimiques
MTHFR protein, human
EC 1.5.1.20
Methylenetetrahydrofolate Reductase (NADPH2)
EC 1.5.1.20
Types de publication
Journal Article
Langues
eng
Pagination
1668-1671Subventions
Organisme : University of Ferrara
ID : FAR-2018
Organisme : Department of Biomedical and Specialty Surgical Sciences, Section of Medical Biochemistry, Molecular Biology and Genetics, University of Ferrara
ID : 62040
Organisme : Department of Biomedical and Specialty Surgical Sciences, Section of Medical Biochemistry, Molecular Biology and Genetics, University of Ferrara
ID : 0039804
Informations de copyright
© 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. All rights reserved.
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