Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases.
Journal
Organic & biomolecular chemistry
ISSN: 1477-0539
Titre abrégé: Org Biomol Chem
Pays: England
ID NLM: 101154995
Informations de publication
Date de publication:
18 06 2019
18 06 2019
Historique:
pubmed:
6
6
2019
medline:
23
1
2020
entrez:
6
6
2019
Statut:
ppublish
Résumé
Utilising a fast and sensitive screening method based on imidazolium-tagged probes, we report unprecedented reversible activity of bacterial β1,4-galactosyltransferases to catalyse the transgalactosylation from lactose to N-acetylglucosamine to form N-acetyllactosamine in the presence of UDP. The process is demonstrated by the preparative scale synthesis of pNP-β-LacNAc from lactose using β1,4-galactosyltransferase NmLgtB-B as the only biocatalyst.
Substances chimiques
Amino Sugars
0
Recombinant Proteins
0
N-acetyllactosamine
3Y5B2K5OOK
Galactosyltransferases
EC 2.4.1.-
Lactose
J2B2A4N98G
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM