Simian Immunodeficiency Virus Infection Modulates CD94


Journal

Journal of virology
ISSN: 1098-5514
Titre abrégé: J Virol
Pays: United States
ID NLM: 0113724

Informations de publication

Date de publication:
15 08 2019
Historique:
received: 10 05 2019
accepted: 03 06 2019
pubmed: 7 6 2019
medline: 4 6 2020
entrez: 7 6 2019
Statut: epublish

Résumé

Recently, we and others have shown that natural killer (NK) cells exhibit memory-like recall responses against cytomegalovirus (CMV) and human immunodeficiency/virus simian immunodeficiency virus (HIV/SIV) infections. Although the mechanism(s) have not been fully delineated, several groups have shown that the activating receptor NKG2C is elevated on NK cells in the context of rhesus CMV (rhCMV) or human CMV (hCMV) infections. CD94, which heterodimerizes with NKG2C is also linked to adaptive NK cell responses. Because nonhuman primates (NHP) play a crucial role in modeling HIV (SIV) infections, it is crucial to be able to assess and characterize the NKG2 family in NHP. Unfortunately, it is not possible to detect CD94 using commercially available antibodies in NHP. Our work, a first for NHP, has focused on developing RNA flow cytometry using mRNA transcripts as proxies distinguishing NKG2C from NKG2A. We have expanded the application of this technology and here we show the first characterization of CD94

Identifiants

pubmed: 31167916
pii: JVI.00731-19
doi: 10.1128/JVI.00731-19
pmc: PMC6675877
pii:
doi:

Substances chimiques

Biomarkers 0
KLRD1 protein, human 0
NK Cell Lectin-Like Receptor Subfamily C 0
NK Cell Lectin-Like Receptor Subfamily D 0

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : NIAID NIH HHS
ID : T32 AI007387
Pays : United States
Organisme : NIAID NIH HHS
ID : P01 AI120756
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI143457
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI120828
Pays : United States
Organisme : NIAID NIH HHS
ID : UM1 AI124377
Pays : United States
Organisme : NIAID NIH HHS
ID : P30 AI060354
Pays : United States

Informations de copyright

Copyright © 2019 American Society for Microbiology.

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Auteurs

Daniel R Ram (DR)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.

Olivier Lucar (O)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.

Brady Hueber (B)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.

R Keith Reeves (RK)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA rreeves@bidmc.harvard.edu.
Ragon Institute of Massachusetts General Hospital, MIT and Harvard, Cambridge, Massachusetts, USA.

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Classifications MeSH