Activation of microglia and macrophages in the circumventricular organs of the mouse brain during TLR2-induced fever and sickness responses.
Brain
Fos
Inflammation
LPS
NF-κB
TLR4
Journal
Journal of neuroimmunology
ISSN: 1872-8421
Titre abrégé: J Neuroimmunol
Pays: Netherlands
ID NLM: 8109498
Informations de publication
Date de publication:
15 09 2019
15 09 2019
Historique:
received:
27
04
2019
revised:
26
05
2019
accepted:
27
05
2019
pubmed:
7
6
2019
medline:
12
6
2020
entrez:
7
6
2019
Statut:
ppublish
Résumé
Toll-like receptor 2 (TLR2) recognizes cell wall components from Gram-positive bacteria. Until now, however, little has been known about the significance of brain TLR2 in controlling inflammation and thermoregulatory responses during systemic Gram-positive bacterial infection. In the present study, the TLR2 immunoreactivity was seen to be prominent in the microglia/macrophages of the circumventricular organs (CVOs) of the mouse brain. The intraperitoneal injection of Pam3CSK4, a TLR2 agonist, induced nuclear factor-κ B activation in the microglia/macrophages of the CVOs. The injection of Pam3CSK4 also produced the expression of Fos at astrocytes and neurons in the CVOs and the regions neighboring the CVOs. The Pam3CSK4 injection induced fever and sickness responses. Pretreatment with lipopolysaccharide, a TLR4 agonist, augmented the Pam3CSK4-induced fever together with the increased TLR2 immunoreactivity. These results indicate that the TLR2 in microglia/macrophages of the CVOs are possibly associated with initiating and transmitting inflammatory responses in the brain.
Identifiants
pubmed: 31170673
pii: S0165-5728(19)30212-7
doi: 10.1016/j.jneuroim.2019.576973
pii:
doi:
Substances chimiques
Lipopeptides
0
Lipopolysaccharides
0
Pam(3)CSK(4) peptide
0
Tlr2 protein, mouse
0
Toll-Like Receptor 2
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
576973Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.