Deletion of Protein Tyrosine Phosphatase Nonreceptor Type 2 in Intestinal Epithelial Cells Results in Upregulation of the Related Phosphatase Protein Tyrosine Phosphatase Nonreceptor Type 23.
HD-PTP
Intestinal epithelial cells
Intestinal inflammation
T cell protein tyrosine phosphatase
Tyrosine phosphatases
Journal
Inflammatory intestinal diseases
ISSN: 2296-9365
Titre abrégé: Inflamm Intest Dis
Pays: Switzerland
ID NLM: 101677990
Informations de publication
Date de publication:
May 2019
May 2019
Historique:
received:
20
09
2018
accepted:
27
02
2019
entrez:
8
6
2019
pubmed:
7
6
2019
medline:
7
6
2019
Statut:
ppublish
Résumé
Knockdown of protein tyrosine phosphatase nonreceptor type 2 ( PTPN2 knockdown in HT-29 cells was induced using siRNA constructs. Acute colitis in PTPN2xVilCre mice was induced by 2% dextran sulfate sodium (DSS) in drinking water for 7 days. Colitis-associated tumors were induced by injection of azoxymethane prior to treatment with DSS for 3 consecutive cycles. In HT-29 cells, Our experiments show that PTPN23 function might, at least partially, compensate lack of PTPN2 in epithelial cells. Upregulation of PTPN23 might therefore crucially contribute to the lack of a colitis phenotype in PTPN2-VilCre mice.
Sections du résumé
BACKGROUND/AIMS
OBJECTIVE
Knockdown of protein tyrosine phosphatase nonreceptor type 2 (
METHODS
METHODS
PTPN2 knockdown in HT-29 cells was induced using siRNA constructs. Acute colitis in PTPN2xVilCre mice was induced by 2% dextran sulfate sodium (DSS) in drinking water for 7 days. Colitis-associated tumors were induced by injection of azoxymethane prior to treatment with DSS for 3 consecutive cycles.
RESULTS
RESULTS
In HT-29 cells,
CONCLUSIONS
CONCLUSIONS
Our experiments show that PTPN23 function might, at least partially, compensate lack of PTPN2 in epithelial cells. Upregulation of PTPN23 might therefore crucially contribute to the lack of a colitis phenotype in PTPN2-VilCre mice.
Identifiants
pubmed: 31172009
doi: 10.1159/000499157
pii: iid-0004-0014
pmc: PMC6537469
doi:
Types de publication
Journal Article
Langues
eng
Pagination
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