A dual functional bone-defect-filling material with sequential antibacterial and osteoinductive properties for infected bone defect repair.
Animals
Anti-Bacterial Agents
/ pharmacology
Bone Morphogenetic Protein 2
/ pharmacology
Bone Regeneration
/ drug effects
Bone and Bones
/ drug effects
Cell Differentiation
/ drug effects
Cell Line
Chitosan
/ analogs & derivatives
Escherichia coli
/ drug effects
Kinetics
Mice
Microbial Sensitivity Tests
Osteogenesis
/ drug effects
Quaternary Ammonium Compounds
/ pharmacology
Rats, Sprague-Dawley
Staphylococcus aureus
/ drug effects
Wound Healing
/ drug effects
antibacterial activity
bone morphogenic protein 2
hydroxypropyltrimethyl ammonium chloride chitosan
infected bone defect healing
osteoinductivity
Journal
Journal of biomedical materials research. Part A
ISSN: 1552-4965
Titre abrégé: J Biomed Mater Res A
Pays: United States
ID NLM: 101234237
Informations de publication
Date de publication:
10 2019
10 2019
Historique:
received:
18
02
2019
revised:
19
05
2019
accepted:
27
05
2019
pubmed:
8
6
2019
medline:
4
9
2020
entrez:
8
6
2019
Statut:
ppublish
Résumé
Infected bone defect healing is hindered by infection and compromised bone regenerative capacity. In this study, we designed a dual functional bone-defect-filling material with a sequential release system, that is, a burst release of a potent antibacterial agent, hydroxypropyltrimethyl ammonium chloride chitosan (HACC), followed by a controlled release of osteoinductive bone morphogenic protein (BMP2) to repair the infected bone defect. Minimum bactericidal concentration (MBC) of HACC against methicillin-resistant Staphylococcus aureus was 40 μg/mL. HACC at 40 μg/mL did not affect preosteoblast proliferation and did not influence the BMP2-induced alkaline phosphatase activity, osteocalcin expression, and matrix mineralization. in vitro release profile revealed burst release of HACC followed by a slow release of BMP2. in vivo bone formation was observed only in the BMP2-containing groups. HACC did not influence of biomimetic calcium phosphate (BioCaP) resorption and BMP2-induced bone formation. In conclusion, the optimized HACC/BMP2-incorporated BioCaP complex showed strong antibacterial effect and robustly enhanced osteoinduction both in vitro and in vivo.
Substances chimiques
Anti-Bacterial Agents
0
Bone Morphogenetic Protein 2
0
Quaternary Ammonium Compounds
0
hydroxypropyltrimethyl ammonium chloride chitosan
0
Chitosan
9012-76-4
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2360-2370Subventions
Organisme : Cultivation and innovation fund of Jinan University
ID : 21617312
Pays : International
Organisme : Guangdong Provincial Science and Technology Plan Project of China
ID : 2017A020211026
Pays : International
Organisme : National Natural Science Foundation of China
ID : 81470724 81400475 81600844 31800808
Pays : International
Organisme : Natural Science Foundation of Hebei Province, China
ID : H2017209238
Pays : International
Organisme : Natural Science Foundation of Zhejiang Province, China
ID : LY14H140002 LY17H140010
Pays : International
Organisme : the funds of ITI project
ID : 868_2012
Pays : International
Informations de copyright
© 2019 Wiley Periodicals, Inc.