Tumor-facing hepatocytes significantly contribute to mild hyperthermia-induced targeting of rat liver metastasis by PLGA-NPs.
Animals
Cadmium Compounds
/ administration & dosage
Colonic Neoplasms
/ metabolism
Drug Carriers
/ administration & dosage
Hepatocytes
/ metabolism
Hyperthermia, Induced
Kupffer Cells
/ metabolism
Liver Neoplasms
/ metabolism
Macrophages
/ metabolism
Male
Nanoparticles
/ administration & dosage
Polylactic Acid-Polyglycolic Acid Copolymer
/ administration & dosage
Rats
Selenium Compounds
/ administration & dosage
Sulfides
/ administration & dosage
Zinc Compounds
/ administration & dosage
Hepatocytes
Liver cancer metastasis
Mild hyperthermia
PLGA-NPs
Tumor associated macrophages
Journal
International journal of pharmaceutics
ISSN: 1873-3476
Titre abrégé: Int J Pharm
Pays: Netherlands
ID NLM: 7804127
Informations de publication
Date de publication:
20 Jul 2019
20 Jul 2019
Historique:
received:
31
01
2019
revised:
22
05
2019
accepted:
03
06
2019
pubmed:
8
6
2019
medline:
18
12
2019
entrez:
8
6
2019
Statut:
ppublish
Résumé
The effect of mild hyperthermia (MHT) on nanoparticle (NP) accumulation in rat model liver metastasis and the contribution of neoplastic and non-neoplastic cells were characterized. CdSe/ZnS QD-doped poly(lactic-co-glycolic acid) (PLGA) NPs (155 ± 10 nm) were delivered via the ileocolic vein to metastatic livers 15 min after localized MW irradiation (1 min, 41 °C) or in normothermia (37 °C, NT). Quantitative analysis of tissue sections by confocal fluorescence microscopy 1 h after NP injection showed no NP tumor accumulation in NT. On the contrary, MHT increased NP association with tumor, compared to normal tissue. Counterstaining of specific markers showed that the MHT effect is due to an increased NP endocytosis not only by tumor cells, but also by hepatocytes at the growing tumor edge and, to a minor extent, by tumor-associated macrophages. High-NP capturing hepatocytes, close to the tumor, may be a relevant phenomenon in MHT-induced increased targeting of NPs to liver metastasis, influencing their therapeutic efficacy.
Identifiants
pubmed: 31173801
pii: S0378-5173(19)30447-8
doi: 10.1016/j.ijpharm.2019.06.004
pii:
doi:
Substances chimiques
Cadmium Compounds
0
Drug Carriers
0
Selenium Compounds
0
Sulfides
0
Zinc Compounds
0
Polylactic Acid-Polyglycolic Acid Copolymer
1SIA8062RS
cadmium selenide
A7F646JC5C
zinc sulfide
KPS085631O
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
541-548Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.