Effects of lipoic acid on primary murine microglial cells.
Actin
Blebs
Cytoskeleton
Microglia
Phagocytosis
Journal
Journal of neuroimmunology
ISSN: 1872-8421
Titre abrégé: J Neuroimmunol
Pays: Netherlands
ID NLM: 8109498
Informations de publication
Date de publication:
15 09 2019
15 09 2019
Historique:
received:
08
03
2019
revised:
09
05
2019
accepted:
24
05
2019
pubmed:
9
6
2019
medline:
12
6
2020
entrez:
9
6
2019
Statut:
ppublish
Résumé
The anti-oxidant lipoic acid (LA) is beneficial in murine models of multiple sclerosis (MS) and has recently been shown to slow brain atrophy in secondary progressive MS. The mechanism of these effects by LA is incompletely understood but may involve effects on microglia. The objective of this study is to understand how LA affects microglial cells. We cultured primary microglial cells from C57BL/6 adult mice brains and stimulated the cells with lipopolysaccharide (LPS) and interferon gamma (IFN-γ) in the presence or absence of LA. We demonstrate the inhibition of phagocytosis, rearrangement of actin, and formation of membrane blebs in stimulated microglia in the presence of LA. These experiments suggest that LA causes changes in microglial actin, which may lead to alterations in phagocytosis, mobility, and migration.
Identifiants
pubmed: 31176014
pii: S0165-5728(19)30106-7
doi: 10.1016/j.jneuroim.2019.576972
pmc: PMC6660368
mid: NIHMS1531190
pii:
doi:
Substances chimiques
Antioxidants
0
Lipopolysaccharides
0
Thioctic Acid
73Y7P0K73Y
Interferon-gamma
82115-62-6
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
576972Subventions
Organisme : NINDS NIH HHS
ID : P30 NS061800
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS057433
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR002369
Pays : United States
Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.
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