Exosomes-mediated transmission of foot-and-mouth disease virus in vivo and in vitro.
Exosomes
FMDV
Immune evasion
Infection
Transmission
Journal
Veterinary microbiology
ISSN: 1873-2542
Titre abrégé: Vet Microbiol
Pays: Netherlands
ID NLM: 7705469
Informations de publication
Date de publication:
Jun 2019
Jun 2019
Historique:
received:
12
02
2019
revised:
24
04
2019
accepted:
24
04
2019
entrez:
10
6
2019
pubmed:
10
6
2019
medline:
27
6
2019
Statut:
ppublish
Résumé
Exosomes are small membrane-enclosed vesicles that participate in intercellular communication between cells. Numerous evidences suggested that exosomes derived from virus-infected cells can mediate virus transmission or/and regulate immune response. Foot-and-mouth disease virus (FMDV) is the prototype member of the Aphthovirus genus of the Picornaviridae family. It can cause highly infectious disease of cloven-hoofed livestock and significantly increase public awareness. However, the role of exosomes in the transmission of FMDV has still remained unknown. In this study, full length of FMDV genomic RNA and partial viral proteins were identified in purified exosomes isolated from FMDV-infected PK-15 cells with qRT-PCR and /MS. Exosomes from FMDV-infected cells were capable of transmitting infection to naive PK-15 cells and suckling mice. Furthermore, exosome-mediated infection cannot be fully blocked by FMDV-specific neutralizing antibodies. This finding highlights that FMDV transmission by exosomes as a potential immune evasion mechanism.
Identifiants
pubmed: 31176404
pii: S0378-1135(19)30197-X
doi: 10.1016/j.vetmic.2019.04.030
pii:
doi:
Substances chimiques
Antibodies, Neutralizing
0
RNA, Viral
0
Viral Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
164-173Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.