Next-Generation Phenotypic Screening in Early Drug Discovery for Infectious Diseases.
black-box complexity
cell-based assay
drug screening
imaging microscopy
Journal
Trends in parasitology
ISSN: 1471-5007
Titre abrégé: Trends Parasitol
Pays: England
ID NLM: 100966034
Informations de publication
Date de publication:
07 2019
07 2019
Historique:
received:
15
02
2019
revised:
08
05
2019
accepted:
08
05
2019
pubmed:
10
6
2019
medline:
29
5
2020
entrez:
10
6
2019
Statut:
ppublish
Résumé
Cell-based phenotypic screening has proven to be valuable, notably in recapitulating relevant biological conditions, for example, the host cell/pathogen niche. However, the corresponding methodological complexity is not readily compatible with high-throughput pipelines, and fails to inform either molecular target or mechanism of action, which frustrates conventional drug-discovery roadmaps. We review the state-of-the-art and emerging technologies that suggest new strategies for harnessing value from the complexity of phenotypic screening and augmenting powerful utility for translational drug discovery. Advances in cellular, molecular, and bioinformatics technologies are converging at a cutting edge where the complexity of phenotypic screening may no longer be considered a hinderance but rather a catalyst to chemotherapeutic discovery for infectious diseases.
Identifiants
pubmed: 31176583
pii: S1471-4922(19)30108-4
doi: 10.1016/j.pt.2019.05.004
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
559-570Informations de copyright
Copyright © 2019 Institut Pasteur. Published by Elsevier Ltd.. All rights reserved.