Side-by-Side Comparison of Skin Biopsies and Skin Tape Stripping Highlights Abnormal Stratum Corneum in Atopic Dermatitis.
Adult
Biomarkers
/ metabolism
Biopsy
/ methods
Cell Differentiation
Child
Dermatitis, Atopic
/ metabolism
Dermatologic Surgical Procedures
/ methods
Epidermis
/ pathology
Filaggrin Proteins
Gene Expression Profiling
Humans
Intercellular Signaling Peptides and Proteins
/ genetics
Intermediate Filament Proteins
/ genetics
Keratin-1
/ genetics
Skin
/ metabolism
Surgical Tape
Journal
The Journal of investigative dermatology
ISSN: 1523-1747
Titre abrégé: J Invest Dermatol
Pays: United States
ID NLM: 0426720
Informations de publication
Date de publication:
11 2019
11 2019
Historique:
received:
17
01
2019
accepted:
26
03
2019
pubmed:
10
6
2019
medline:
9
6
2020
entrez:
10
6
2019
Statut:
ppublish
Résumé
Skin biopsies are commonly used for the assessment of skin pathology in various skin diseases, including atopic dermatitis (AD). However, because of the invasive nature of skin biopsies, many patients, particularly children, decline participation. This can lead to potential subject sampling bias as data could be skewed toward more severe, older patients who are willing to have biopsies. Recently, researchers have begun studying the skin with a minimal, noninvasive technique using skin tape stripping (STS) to profile the epidermal transcriptome, proteins, and lipids in the skin. However, side-by-side comparisons of skin biopsies with STS have not been done to assess epidermal penetration. Therefore, 20 STS were collected from the volar surface of forearms from healthy nonatopic subjects and patients with AD, following this skin biopsies were collected from adjacent nontaped and taped areas of the skin. Using hematoxylin and eosin staining and immunostaining, we demonstrated that 20 STS reached the upper granular layer of the epidermis. Additionally, we found that the expression of terminal differentiation markers in samples from STS procedure positively correlated with the expression level of these markers in matching skin biopsies. Therefore, STS is a noninvasive and reliable approach to evaluate the expression of skin terminal differentiation markers, which are defective in AD skin.
Identifiants
pubmed: 31176708
pii: S0022-202X(19)31632-X
doi: 10.1016/j.jid.2019.03.1160
pmc: PMC6814531
mid: NIHMS1531243
pii:
doi:
Substances chimiques
Biomarkers
0
CDSN protein, human
0
Filaggrin Proteins
0
Intercellular Signaling Peptides and Proteins
0
Intermediate Filament Proteins
0
Keratin-1
0
Types de publication
Comparative Study
Letter
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2387-2389.e1Subventions
Organisme : NIAMS NIH HHS
ID : R01 AR041256
Pays : United States
Organisme : NIAID NIH HHS
ID : U01 AI147462
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR002535
Pays : United States
Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.
Références
JCI Insight. 2018 Feb 22;3(4):
pubmed: 29467325
J Allergy Clin Immunol. 2009 Nov;124(5):1113-5.e1-11
pubmed: 19748658
Allergy. 2011 Jul;66(7):934-40
pubmed: 21261659
J Invest Dermatol. 2016 Nov;136(11):2192-2200
pubmed: 27381887
J Immunol. 2003 Sep 15;171(6):3262-9
pubmed: 12960356
J Am Acad Dermatol. 2016 Jun;74(6):1086-92
pubmed: 26951940
Br J Dermatol. 2017 Feb;176(2):537-540
pubmed: 27572518
Ann Allergy Asthma Immunol. 2018 Jan;120(1):34-41
pubmed: 29126710
J Allergy Clin Immunol. 2012 Dec;130(6):1344-54
pubmed: 22951056
Br J Dermatol. 2019 Mar;180(3):586-596
pubmed: 30132823
J Allergy Clin Immunol. 2018 Apr;141(4):1298-1309
pubmed: 29309794
J Allergy Clin Immunol. 2016 Apr;137(4):1282-1285.e4
pubmed: 26879860
J Invest Dermatol. 2018 Oct;138(10):2224-2233
pubmed: 29604251
Nat Rev Mol Cell Biol. 2005 Apr;6(4):328-40
pubmed: 15803139
Br J Dermatol. 2017 Oct;177(4):e125-e127
pubmed: 28244066