Genetic and virulence characterization of colistin-resistant and colistin-sensitive A. baumannii clinical isolates.
Acinetobacter Infections
/ microbiology
Acinetobacter baumannii
/ drug effects
Animals
Anti-Bacterial Agents
/ pharmacology
Bacterial Proteins
/ genetics
Colistin
/ pharmacology
Disease Models, Animal
Drug Resistance, Bacterial
/ genetics
Gene Expression Regulation, Bacterial
Genome, Bacterial
/ genetics
Humans
Microbial Sensitivity Tests
Moths
Mutation
Transcription Factors
/ genetics
Virulence
/ genetics
Acinetobacter baumannii
Galleria mellonella
colistin-resistant
resistance
virulence
whole-sequencing
Journal
Diagnostic microbiology and infectious disease
ISSN: 1879-0070
Titre abrégé: Diagn Microbiol Infect Dis
Pays: United States
ID NLM: 8305899
Informations de publication
Date de publication:
Sep 2019
Sep 2019
Historique:
received:
08
01
2019
revised:
29
03
2019
accepted:
24
04
2019
pubmed:
11
6
2019
medline:
18
12
2019
entrez:
11
6
2019
Statut:
ppublish
Résumé
Treatment of infections caused by A. baumannii is becoming a challenge due to the ability to develop multidrug-resistance, virulence, and high mortality. We described the colistin resistance and virulence genes present in sixA. baumannii clinical isolates using WGS, expression by qPCR, and virulence in the Galleria mellonella model. The colistin-resistant isolates were assigned as ST233 and the colistin-susceptible isolates as ST236 and ST407. The colistin-resistant isolates contained mutations within PmrA/PmrB, and the pmrA showed up-regulation in all of them. Only one colistin-resistant isolate indicating virulence in G. mellonella. This particular isolate belonged to a different clone, and it was the only isolate that presented non-synonymous mutations in pmrB. Colistinresistance in A. baumannii isolates seems to be caused by up-regulation of pmrA gene. Only one isolate appeared to be virulent in the G. mellonella model. This finding indicating low virulence in isolates belonging to emerging clones circulating in our hospital.
Identifiants
pubmed: 31178071
pii: S0732-8893(19)30018-5
doi: 10.1016/j.diagmicrobio.2019.04.013
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Bacterial Proteins
0
PmrB protein, bacteria
0
Transcription Factors
0
pmrA protein, Bacteria
0
Colistin
Z67X93HJG1
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
99-101Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.