Switching the Post-translational Modification of Translation Elongation Factor EF-P.

EpmA EarP IF5A NleB Pseudomonas aeruginosa TDP-rhamnose bacterial two-hybrid glycosylation

Journal

Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977

Informations de publication

Date de publication:
2019
Historique:
received: 30 01 2019
accepted: 06 05 2019
entrez: 11 6 2019
pubmed: 11 6 2019
medline: 11 6 2019
Statut: epublish

Résumé

Tripeptides with two consecutive prolines are the shortest and most frequent sequences causing ribosome stalling. The bacterial translation elongation factor P (EF-P) relieves this arrest, allowing protein biosynthesis to continue. A seven amino acids long loop between beta-strands β3/β4 is crucial for EF-P function and modified at its tip by lysylation of lysine or rhamnosylation of arginine. Phylogenetic analyses unveiled an invariant proline in the -2 position of the modification site in EF-Ps that utilize lysine modifications such as

Identifiants

pubmed: 31178848
doi: 10.3389/fmicb.2019.01148
pmc: PMC6544042
doi:

Types de publication

Journal Article

Langues

eng

Pagination

1148

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Auteurs

Wolfram Volkwein (W)

Center for Integrated Protein Science Munich, Department of Biology I, Microbiology, Ludwig-Maximilians-Universität München, Munich, Germany.

Ralph Krafczyk (R)

Center for Integrated Protein Science Munich, Department of Biology I, Microbiology, Ludwig-Maximilians-Universität München, Munich, Germany.

Pravin Kumar Ankush Jagtap (PKA)

Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.

Marina Parr (M)

Department of Bioinformatics, Wissenschaftszentrum Weihenstephan, Technische Universität München, Freising, Germany.
St. Petersburg State Polytechnic University, Saint Petersburg, Russia.

Elena Mankina (E)

Department of Bioinformatics, Wissenschaftszentrum Weihenstephan, Technische Universität München, Freising, Germany.

Jakub Macošek (J)

Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
Faculty of Biosciences, Collaboration for Joint PhD Degree Between EMBL and Heidelberg University, Heidelberg, Germany.

Zhenghuan Guo (Z)

Center for Integrated Protein Science Munich, Department of Biology I, Microbiology, Ludwig-Maximilians-Universität München, Munich, Germany.

Maximilian Josef Ludwig Johannes Fürst (MJLJ)

Center for Integrated Protein Science Munich, Department of Biology I, Microbiology, Ludwig-Maximilians-Universität München, Munich, Germany.
Molecular Enzymology Group, University of Groningen, Groningen, Netherlands.

Miriam Pfab (M)

Center for Integrated Protein Science Munich, Department of Biology I, Microbiology, Ludwig-Maximilians-Universität München, Munich, Germany.

Dmitrij Frishman (D)

Department of Bioinformatics, Wissenschaftszentrum Weihenstephan, Technische Universität München, Freising, Germany.
St. Petersburg State Polytechnic University, Saint Petersburg, Russia.

Janosch Hennig (J)

Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.

Kirsten Jung (K)

Center for Integrated Protein Science Munich, Department of Biology I, Microbiology, Ludwig-Maximilians-Universität München, Munich, Germany.

Jürgen Lassak (J)

Center for Integrated Protein Science Munich, Department of Biology I, Microbiology, Ludwig-Maximilians-Universität München, Munich, Germany.

Classifications MeSH