Hepatitis B virus reverse transcriptase polymorphisms between treated and treatment-naïve chronically infected patients.
Chronic hepatitis B virus
Drug resistance conferring substitution
HBV reverse transcriptase
Hepatitis B virus polymerase
Nucleotide analogues resistance mutation
Journal
Virusdisease
ISSN: 2347-3584
Titre abrégé: Virusdisease
Pays: India
ID NLM: 101624144
Informations de publication
Date de publication:
Jun 2019
Jun 2019
Historique:
received:
04
11
2018
accepted:
29
12
2018
entrez:
11
6
2019
pubmed:
11
6
2019
medline:
11
6
2019
Statut:
ppublish
Résumé
The aim of this study was investigation of variation(s) in the Hepatitis B virus (HBV) reverse transcriptase domain. 120 patients with chronic HBV infection recruited. 104 patients were received nucleos(t)ide analogs treatments. DNA extractions were done from plasma samples. Direct sequencing and alignment of Polymerase Chain Reaction products were applied for further analysis. HBV genotypes determined by NCBI's Genotyping Tool. Polymorphism(s) were detected by using DnaSP software. Of 120 samples, 98 were sequenced. All of products were HBV genotype D. 13/98 (13.27%) of patients had M539I/V substitutions corresponding to YMDD motif. FLLAQ to FLMAQ was observed among 22/98 (22.98) patients. Two substitutions N459Y and L515M were significantly correlated (R
Identifiants
pubmed: 31179360
doi: 10.1007/s13337-018-00510-5
pii: 510
pmc: PMC6531556
doi:
Types de publication
Journal Article
Langues
eng
Pagination
219-226Déclaration de conflit d'intérêts
Conflict of interestThe authors have no conflict of interest to declare.
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