Rapid detection and molecular survey of blaVIM, blaIMP and blaNDM genes among clinical isolates of Acinetobacter baumannii using new multiplex real-time PCR and melting curve analysis.
Acinetobacter baumannii
Melting curve analysis
Multiplex real-time PCR
Single tube reaction
Journal
BMC microbiology
ISSN: 1471-2180
Titre abrégé: BMC Microbiol
Pays: England
ID NLM: 100966981
Informations de publication
Date de publication:
10 06 2019
10 06 2019
Historique:
received:
13
05
2018
accepted:
03
06
2019
entrez:
12
6
2019
pubmed:
12
6
2019
medline:
4
4
2020
Statut:
epublish
Résumé
Acinetobacter baumannii is a cosmopolitan bacterium that is frequently reported from hospitalized patients, especially those patients who admitted in the intensive care unit. Recently, multiplex real-time PCR has been introduced for rapid detection of the resistance genes in clinical isolates of bacteria. The current study aimed to develop and evaluate multiplex real-time PCR to detect common resistance genes among clinical isolates of A. baumannii. Multiplex real-time PCR based on melting curve analysis showed different T The current study showed that blaVIM, was the most prevalent MBL gene among the clinical isolates of A. baumannii while no amplification of blaNDM was seen. Multiplex real-time PCR can be sensitive and reliable technique for rapid detection of resistance genes in clinical isolates.
Sections du résumé
BACKGROUND
Acinetobacter baumannii is a cosmopolitan bacterium that is frequently reported from hospitalized patients, especially those patients who admitted in the intensive care unit. Recently, multiplex real-time PCR has been introduced for rapid detection of the resistance genes in clinical isolates of bacteria. The current study aimed to develop and evaluate multiplex real-time PCR to detect common resistance genes among clinical isolates of A. baumannii.
RESULTS
Multiplex real-time PCR based on melting curve analysis showed different T
CONCLUSIONS
The current study showed that blaVIM, was the most prevalent MBL gene among the clinical isolates of A. baumannii while no amplification of blaNDM was seen. Multiplex real-time PCR can be sensitive and reliable technique for rapid detection of resistance genes in clinical isolates.
Identifiants
pubmed: 31182026
doi: 10.1186/s12866-019-1510-y
pii: 10.1186/s12866-019-1510-y
pmc: PMC6558830
doi:
Substances chimiques
Bacterial Proteins
0
beta-Lactamases
EC 3.5.2.6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
122Références
PLoS One. 2017 Jun 21;12(6):e0179651
pubmed: 28636666
PLoS One. 2014 Jul 17;9(7):e100956
pubmed: 25033234
Int J Microbiol. 2017;2017:3925868
pubmed: 28567057
PLoS One. 2012;7(12):e52452
pubmed: 23300673
Microb Drug Resist. 2018 Mar;24(2):136-141
pubmed: 28691891
Scientifica (Cairo). 2014;2014:245162
pubmed: 25133013
Antimicrob Resist Infect Control. 2015 Jan 29;4(1):2
pubmed: 25648393
J Glob Antimicrob Resist. 2017 Sep;10:88-94
pubmed: 28711559
Clin Microbiol Infect. 2006 Sep;12(9):826-36
pubmed: 16882287
J Infect Chemother. 2017 Sep;23(9):655-657
pubmed: 28408304
PLoS One. 2016 Jul 08;11(7):e0158958
pubmed: 27391234
Clin Infect Dis. 2017 May 15;64(suppl_2):S68-S75
pubmed: 28475792
J Microbiol Methods. 2015 Jun;113:4-9
pubmed: 25819308
J Antimicrob Chemother. 2012 Apr;67(4):906-9
pubmed: 22232516
Afr J Infect Dis. 2014;8(1):14-8
pubmed: 24653812
J Clin Microbiol. 2013 Apr;51(4):1247-9
pubmed: 23325823
Crit Care Clin. 2008 Apr;24(2):237-48, vii
pubmed: 18361943
Saudi J Biol Sci. 2015 Jul;22(4):424-9
pubmed: 26150748
Chemotherapy. 2016;61(5):275-80
pubmed: 27058056
Ann Clin Microbiol Antimicrob. 2013 Oct 22;12:30
pubmed: 24143953
ACS Omega. 2017 Oct 31;2(10):7400-7409
pubmed: 30023551
Expert Opin Pharmacother. 2014 Jul;15(10):1351-70
pubmed: 24766095
Ann Clin Microbiol Antimicrob. 2017 Jul 19;16(1):51
pubmed: 28724383
J Med Microbiol. 2017 Jul;66(7):999-1004
pubmed: 28671522
Clin Microbiol Rev. 2017 Jan;30(1):409-447
pubmed: 27974412
J Hosp Infect. 2017 Aug;96(4):323-327
pubmed: 28532975
J Antimicrob Chemother. 2013 Jul;68(7):1572-5
pubmed: 23435692
J Hosp Infect. 2016 Jan;92(1):67-72
pubmed: 26601601
Int J Antimicrob Agents. 2016 Jul;48(1):46-50
pubmed: 27216382
N Engl J Med. 2008 Mar 20;358(12):1271-81
pubmed: 18354105
Curr Top Microbiol Immunol. 2016;398:3-33
pubmed: 27406189
Expert Rev Anti Infect Ther. 2015 May;13(5):567-73
pubmed: 25850806
J Clin Microbiol. 2007 Feb;45(2):544-7
pubmed: 17093019
Microorganisms. 2017 Apr 12;5(2):
pubmed: 28417918
Mayo Clin Proc. 2012 Feb;87(2):198-208
pubmed: 22305032
Expert Rev Anti Infect Ther. 2008 Jun;6(3):309-25
pubmed: 18588496