Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
11 06 2019
11 06 2019
Historique:
received:
15
11
2018
accepted:
23
05
2019
entrez:
13
6
2019
pubmed:
13
6
2019
medline:
21
10
2020
Statut:
epublish
Résumé
Mitochondria are key for cellular metabolism and signalling processes during viral infection. We report a methodology to analyse mitochondrial properties at the single-organelle level during viral infection using a recombinant adenovirus coding for a mitochondrial tracer protein for tagging and detection by multispectral flow cytometry. Resolution at the level of tagged individual mitochondria revealed changes in mitochondrial size, membrane potential and displayed a fragile phenotype during viral infection of cells. Thus, single-organelle and multi-parameter resolution allows to explore altered energy metabolism and antiviral defence by tagged mitochondria selectively in virus-infected cells and will be instrumental to identify viral immune escape and to develop and monitor novel mitochondrial-targeted therapies.
Identifiants
pubmed: 31186476
doi: 10.1038/s41598-019-44922-9
pii: 10.1038/s41598-019-44922-9
pmc: PMC6560178
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
8492Références
J Allergy Clin Immunol. 2013 Mar;131(3):815-24
pubmed: 23141740
Methods Cell Biol. 2001;64:117-28
pubmed: 11070835
Hepatology. 2018 Dec;68(6):2089-2105
pubmed: 29729204
J Neurosci. 2012 Nov 14;32(46):16203-12
pubmed: 23152604
React Oxyg Species (Apex). 2017 Jul;4(10):275-283
pubmed: 29806036
Biochim Biophys Acta. 2013 Sep;1828(9):2121-33
pubmed: 23685124
Curr Protoc Cell Biol. 2010 Mar;Chapter 4:Unit 4.25.1-21
pubmed: 20235105
Exp Cell Res. 2004 Mar 10;294(1):106-17
pubmed: 14980506
Hepatology. 2010 Apr;51(4):1430-7
pubmed: 20373369
Cell Rep. 2012 Sep 27;2(3):478-87
pubmed: 22939982
Carcinogenesis. 2014 Jul;35(7):1592-601
pubmed: 24632494
Anal Biochem. 2013 Dec 1;443(1):66-74
pubmed: 23969012
Cytometry A. 2004 Aug;60(2):145-54
pubmed: 15290715
PLoS Pathog. 2013 Mar;9(3):e1003285
pubmed: 23555273
Semin Thromb Hemost. 2010 Nov;36(8):807-18
pubmed: 21049381
J Cell Biol. 1969 Jul;42(1):68-91
pubmed: 4891915
Cell. 2005 Sep 9;122(5):669-82
pubmed: 16125763
J Immunol. 2014 Sep 15;193(6):3080-9
pubmed: 25135833
Proc Natl Acad Sci U S A. 2014 Apr 29;111(17):6413-8
pubmed: 24733894
Cell. 2006 Jun 30;125(7):1241-52
pubmed: 16814712
Circ Res. 2012 Oct 12;111(9):1198-207
pubmed: 23065343
Eur J Cell Biol. 2018 Jan;97(1):1-14
pubmed: 29092745
FEBS Lett. 2002 Jan 16;510(3):127-32
pubmed: 11801239
Sci Rep. 2018 Nov 5;8(1):16354
pubmed: 30397207
Mol Cell Biochem. 2004 Jul;262(1-2):1-16
pubmed: 15532704
Nat Med. 2014 May;20(5):555-60
pubmed: 24747747
Mitochondrion. 2014 Nov;19 Pt A:113-23
pubmed: 24969531
Biochim Biophys Acta. 2010 Jun-Jul;1797(6-7):907-12
pubmed: 20079335
PLoS Pathog. 2013;9(12):e1003722
pubmed: 24339771
J Mol Biol. 2013 Dec 13;425(24):5009-19
pubmed: 24120683
Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):11984-9
pubmed: 11050229
Methods Mol Biol. 2015;1295:87-97
pubmed: 25820716
Nat Commun. 2018 Nov 15;9(1):4805
pubmed: 30442932
Nat Rev Immunol. 2012 Feb 24;12(3):201-13
pubmed: 22362353
J Virol. 2014 May;88(9):5152-64
pubmed: 24574393
Rev Sci Instrum. 1969 Aug;40(8):1029-32
pubmed: 5797881