Tumor growth under rhGM-CSF application in an orthotopic rodent glioma model.
glioma
macrophages
microglia
orthotopic rodent glioma model
recombinant human granulocyte macrophage colony stimulating factor
Journal
Oncology letters
ISSN: 1792-1074
Titre abrégé: Oncol Lett
Pays: Greece
ID NLM: 101531236
Informations de publication
Date de publication:
Jun 2019
Jun 2019
Historique:
received:
20
09
2018
accepted:
14
02
2019
entrez:
13
6
2019
pubmed:
13
6
2019
medline:
13
6
2019
Statut:
ppublish
Résumé
Regulation of the host immune response serves a pivotal role in the persistence and progression of malignant glioma. To date, cytotoxic cluster of differentiation (CD)-8+ T and natural killer cells are considered the main cellular components of host tumor control. The influence of macrophages in an orthotropic C6 tumor implantation model was investigated and the aim of the present study was to characterize the effects of systemic macrophage-activation on glioma growth by using the granulocyte macrophage colony stimulating factor (rhGM-CSF). A total of 20 male Sprague-Dawley rats were orthotopically implanted with C6 glioma spheroids and treated subcutaneously with 10 µg/kg rhGM-CSF every other day; 9 animals served as controls. Serial magnetic resonance imaging was performed on days 7, 14, 21, 28, 32 and 42 post-implantation to monitor tumor volume. Histological work-up included hematoxylin and eosin, CD68/ED-1 macrophage, CD8 T-cell and Ki-67 MIB1 proliferation staining in gliomas and spleen. Experimental C6-gliomas developed in 15/20 (75%) animals. In rhGM-CSF treated rats, tumors developed significantly later and reached a smaller size (median, 134 mm
Identifiants
pubmed: 31186691
doi: 10.3892/ol.2019.10179
pii: OL-0-0-10179
pmc: PMC6507467
doi:
Types de publication
Journal Article
Langues
eng
Pagination
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