Inhibitory activity of chitosan nanoparticles against Cryptosporidium parvum oocysts.
Activity
Bioassay
Chitosan
Cryptosporidium
In vitro
Nanoparticles
Journal
Parasitology research
ISSN: 1432-1955
Titre abrégé: Parasitol Res
Pays: Germany
ID NLM: 8703571
Informations de publication
Date de publication:
Jul 2019
Jul 2019
Historique:
received:
01
02
2019
accepted:
24
05
2019
pubmed:
13
6
2019
medline:
11
10
2019
entrez:
13
6
2019
Statut:
ppublish
Résumé
Cryptosporidium is a ubiquitous harsh protozoan parasite that resists many disinfectants. It remains viable and infective for a long time in water and food causing global outbreaks. Chitosan (the deacetylated chitin molecule) was used in its nanosuspension form to evaluate its effect against Cryptosporidium parvum. The experiments were performed in vitro in serial concentrations and confirmed in mice in vivo infectivity assay. Chitosan nanoparticles (Cs NPs) were toxic to Cryptosporidium oocysts. The effect appeared to decrease the number of Cryptosporidium oocysts and altered their content. The destruction rate of oocysts was dependent on the dose of chitosan and the time of exposure (P < 0.05). Higher doses of Cs NPs over a prolonged period exhibited a significantly higher destruction rate. Using staining and light microscopy, remarkable destructive changes were observed in the oocysts' morphology. The minimal lethal dose for > 90% of oocysts was 3000 μg/ml, no mice infections in vivo were observed. The results in this study elucidate Cs NPs as an effective anti-cryptosporidial agent.
Identifiants
pubmed: 31187224
doi: 10.1007/s00436-019-06364-0
pii: 10.1007/s00436-019-06364-0
doi:
Substances chimiques
Antiprotozoal Agents
0
Chitosan
9012-76-4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2053-2063Références
FEMS Microbiol Lett. 1999 Sep 15;178(2):227-33
pubmed: 10499272
Pharm Res. 1999 Oct;16(10):1576-81
pubmed: 10554100
J Eukaryot Microbiol. 2000 May-Jun;47(3):294-8
pubmed: 10847347
Int J Pharm. 2001 Feb 19;214(1-2):83-5
pubmed: 11282242
Diagn Microbiol Infect Dis. 2002 Aug;43(4):283-8
pubmed: 12151188
Carbohydr Res. 2004 Nov 15;339(16):2693-700
pubmed: 15519328
Eur J Pharm Sci. 2005 Jul-Aug;25(4-5):427-37
pubmed: 15893461
Appl Environ Microbiol. 2006 Sep;72(9):6242-7
pubmed: 16957251
Lett Appl Microbiol. 2006 Nov;43(5):475-81
pubmed: 17032219
J Water Health. 2007 Mar;5(1):1-38
pubmed: 17402277
Trends Parasitol. 2007 Aug;23(8):344-7
pubmed: 17581791
Med Princ Pract. 2008;17(2):89-101
pubmed: 18287791
Int J Food Microbiol. 2009 Jun 30;132(2-3):127-33
pubmed: 19439383
Vet Parasitol. 2009 Nov 12;165(3-4):187-99
pubmed: 19660869
Int J Food Microbiol. 2010 Nov 15;144(1):51-63
pubmed: 20951455
Chem Pharm Bull (Tokyo). 2010 Nov;58(11):1423-30
pubmed: 21048331
Infect Immun. 1990 Apr;58(4):961-9
pubmed: 2108092
Int J Nanomedicine. 2011;6:765-74
pubmed: 21589644
Water Res. 2011 Dec 15;45(20):6603-14
pubmed: 22048017
Parasitol Res. 2012 Aug;111(2):545-54
pubmed: 22392135
Vet Parasitol. 2012 Sep 10;188(3-4):203-14
pubmed: 22521978
Int J Pharm. 2012 Sep 15;434(1-2):292-305
pubmed: 22664460
Clin Infect Dis. 2012 Dec;55 Suppl 4:S232-45
pubmed: 23169936
Int J Biomater. 2013;2013:146320
pubmed: 24194759
Carbohydr Polym. 2014 Jan 30;101:1101-8
pubmed: 24299880
Exp Parasitol. 2014 Aug;143:30-8
pubmed: 24852215
Dose Response. 2013 Nov 07;12(2):202-32
pubmed: 24910581
Lancet Infect Dis. 2015 Jan;15(1):85-94
pubmed: 25278220
Rev Environ Contam Toxicol. 2015;233:1-43
pubmed: 25367132
PLoS Pathog. 2015 Jun 25;11(6):e1004942
pubmed: 26110623
Environ Sci Technol. 2015 Nov 3;49(21):12958-67
pubmed: 26398590
Appl Environ Microbiol. 2015 Oct 23;82(2):431-7
pubmed: 26497464
Carbohydr Polym. 2016 Jan 20;136:682-91
pubmed: 26572401
Clin Microbiol Infect. 2016 Jun;22(6):471-80
pubmed: 27172805
Parasite. 2017;24:1
pubmed: 28098070
Water Res. 2017 May 1;114:14-22
pubmed: 28214721
Nat Rev Drug Discov. 2017 Jul 31;16(8):527
pubmed: 28757629
Br J Biomed Sci. 2018 Jan;75(1):13-18
pubmed: 28945174
Parasit Vectors. 2017 Oct 23;10(1):509
pubmed: 29061171
Acta Trop. 2018 Feb;178:73-80
pubmed: 29092797
Int J Parasitol. 2018 Jan;48(1):1-12
pubmed: 29122606
Pharmaceutics. 2017 Nov 20;9(4):null
pubmed: 29156634
Parasitol Res. 2018 Mar;117(3):629-653
pubmed: 29350281
J Food Sci Technol. 2018 Mar;55(3):1127-1133
pubmed: 29487455
J Parasit Dis. 2018 Mar;42(1):102-113
pubmed: 29491568
Int J Environ Res Public Health. 2018 Mar 12;15(3):
pubmed: 29534511
Int J Nanomedicine. 2018 Mar 08;13:1341-1351
pubmed: 29563791
Int J Hyg Environ Health. 2018 Jun;221(5):743-763
pubmed: 29776848
Parasitol Int. 2018 Dec;67(6):666-674
pubmed: 29933042
Exp Parasitol. 2018 Nov;194:1-8
pubmed: 30237052
Pathog Glob Health. 2018 Sep;112(6):281-293
pubmed: 30332341
Int J Biol Macromol. 2019 Apr 1;126:262-272
pubmed: 30584935
Int J Biol Macromol. 2019 Apr 1;126:731-742
pubmed: 30593811
Eur J Protistol. 2019 Jun;69:70-87
pubmed: 30981203
Acta Vet Scand. 1981;22(3-4):594-6
pubmed: 6178277
J Infect Dis. 1983 May;147(5):824-8
pubmed: 6842020
J Parasitol. 1994 Oct;80(5):816-9
pubmed: 7931917
Appl Environ Microbiol. 1993 Nov;59(11):3661-5
pubmed: 8285673