Claudin-15 forms a water channel through the tight junction with distinct function compared to claudin-2.
claudin-15 pore diameter
paracellular ion permeability
paracellular water permeability
Journal
Acta physiologica (Oxford, England)
ISSN: 1748-1716
Titre abrégé: Acta Physiol (Oxf)
Pays: England
ID NLM: 101262545
Informations de publication
Date de publication:
01 2020
01 2020
Historique:
received:
04
12
2018
revised:
06
06
2019
accepted:
07
06
2019
pubmed:
13
6
2019
medline:
9
1
2021
entrez:
13
6
2019
Statut:
ppublish
Résumé
Claudin-15 is mainly expressed in the small intestine and indirectly involved in glucose absorption. Similar to claudin-2 and -10b, claudin-15 is known to form a paracellular channel for small cations. Claudin-2, but not claudin-10b, also forms water channels. Here we experimentally tested whether claudin-15 also mediates water transport and if yes, whether water transport is Na MDCK C7 cells were stably transfected with claudin-15. Ion and water permeability were investigated in confluent monolayers of control and claudin-15-expressing cells. Water flux was induced by an osmotic or ionic gradient. Expression of claudin-15 in MDCK cells strongly increased cation permeability. The permeability ratios for monovalent cations indicated a passage of partially hydrated ions through the claudin-15 pore. Accordingly, its pore diameter was determined to be larger than that of claudin-2 and claudin-10b. Mannitol-induced water flux was elevated in claudin-15-expressing cells compared to control cells. In contrast to the Na Claudin-15, similar to claudin-2, forms a paracellular cation and water channel. In functional contrast to claudin-2, water and Na
Substances chimiques
Aquaporins
0
Claudin-2
0
Claudins
0
Tight Junction Proteins
0
claudin 15
0
Water
059QF0KO0R
Sodium
9NEZ333N27
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13334Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : FR 652/12-1
Pays : International
Organisme : Deutsche Forschungsgemeinschaft
ID : GRK 2318
Pays : International
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2019 Scandinavian Physiological Society. Published by John Wiley & Sons Ltd.
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