A modified exponential amplification reaction (EXPAR) with an improved signal-to-noise ratio for ultrasensitive detection of polynucleotide kinase.

Biosensing Techniques HeLa Cells Humans Nucleic Acid Amplification Techniques Polynucleotide 5'-Hydroxyl-Kinase / analysis Signal-To-Noise Ratio Spectrometry, Fluorescence

Journal

Chemical communications (Cambridge, England)
ISSN: 1364-548X
Titre abrégé: Chem Commun (Camb)
Pays: England
ID NLM: 9610838

Informations de publication

Date de publication:
07 Jul 2019
Historique:
pubmed: 14 6 2019
medline: 12 7 2019
entrez: 14 6 2019
Statut: ppublish

Résumé

We applied a modified exponential amplification reaction (EXPAR) strategy to design a label-free and "one-pot" biosensor for ultrasensitive detection of polynucleotide kinase (PNK). This method was also successfully applied in the screening of PNK inhibitors and analysis of the endogenous PNK activity at the single-cell level.

Identifiants

DOI: 10.1039/c9cc03568k PMID: 31192323
pubmed: 31192323
doi: 10.1039/c9cc03568k
doi:

Substances chimiques

Polynucleotide 5'-Hydroxyl-Kinase EC 2.7.1.78

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

7611-7614

Auteurs

Yu-Peng Zhang (YP)

State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin, 300071, P. R. China. kongdem@nankai.edu.cn.

Yun-Xi Cui (YX)

State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin, 300071, P. R. China. kongdem@nankai.edu.cn.
ORCID: 0000-0002-3830-3336

Xiao-Yu Li (XY)

State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin, 300071, P. R. China. kongdem@nankai.edu.cn.

Yi-Chen Du (YC)

State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin, 300071, P. R. China. kongdem@nankai.edu.cn.

An-Na Tang (AN)

State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin, 300071, P. R. China. kongdem@nankai.edu.cn.
ORCID: 0000-0001-5411-9216

De-Ming Kong (DM)

State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin, 300071, P. R. China. kongdem@nankai.edu.cn and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300071, P. R. China.
ORCID: 0000-0002-9216-8040

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Techniques de biocapteur › A modified exponential amplification reaction...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules HeLa › A modified exponential amplification reaction...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › A modified exponential amplification reaction...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › A modified exponential amplification reaction...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Phosphotransferases › Phosphotransferases (Alcohol Group Acceptor) › Polynucleotide 5'-hydroxyl-kinase › A modified exponential amplification reaction...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Statistiques comme sujet › Sensibilité et spécificité › Rapport signal-bruit › A modified exponential amplification reaction...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › Spectrométrie de fluorescence › A modified exponential amplification reaction...