Essential long-range action of Wingless/Wnt in adult intestinal compartmentalization.
Animals
Body Patterning
/ genetics
Cell Differentiation
/ genetics
Cell Lineage
/ genetics
Cell Proliferation
/ genetics
Drosophila Proteins
/ genetics
Drosophila melanogaster
/ genetics
Epithelial Cells
/ cytology
Gene Expression Regulation, Developmental
/ genetics
Homeostasis
Intestines
/ growth & development
Signal Transduction
/ genetics
Stem Cells
/ metabolism
Wnt1 Protein
/ genetics
Journal
PLoS genetics
ISSN: 1553-7404
Titre abrégé: PLoS Genet
Pays: United States
ID NLM: 101239074
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
12
07
2018
accepted:
26
03
2019
entrez:
14
6
2019
pubmed:
14
6
2019
medline:
7
11
2019
Statut:
epublish
Résumé
Signal transduction activated by Wingless/Wnt ligands directs cell proliferation and fate specification in metazoans, and its overactivation underlies the development of the vast majority of colorectal cancers. In the conventional model, the secretion and movement of Wingless to cells distant from its source of synthesis are essential for long-range signaling in tissue patterning. However, this model was upended recently by an unanticipated finding: replacement of wild-type Drosophila Wingless with a membrane-tethered form produced viable adults with largely normal external morphology, which suggested that Wingless secretion and movement are dispensable for tissue patterning. Herein, we tested this foundational principle in the adult intestine, where Wingless signaling gradients coincide with all major boundaries between compartments. We find that the critical roles of Wingless during adult intestinal development, which include regulation of target gene activation, boundary formation, stem cell proliferation, epithelial cell fate specification, muscle differentiation, gut folding, and signaling crosstalk with the Decapentaplegic pathway, are all disrupted by Wingless tethering. These findings provide new evidence that supports the requirement for the direct, long-range action of Wingless in tissue patterning, with relevance for animal development, tissue homeostasis and Wnt-driven disease.
Identifiants
pubmed: 31194729
doi: 10.1371/journal.pgen.1008111
pii: PGENETICS-D-18-01420
pmc: PMC6563961
doi:
Substances chimiques
Drosophila Proteins
0
Wnt1 Protein
0
dpp protein, Drosophila
0
wg protein, Drosophila
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1008111Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM122222
Pays : United States
Organisme : NIGMS NIH HHS
ID : P20 GM113132
Pays : United States
Organisme : NIH HHS
ID : P40 OD018537
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA023108
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM121421
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM084947
Pays : United States
Commentaires et corrections
Type : CommentIn
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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