Two open-label, single arm, non-randomized phase II studies of irinotecan for the treatment of metastatic breast cancer in patients with increased copy number of the topoisomerase I gene.


Journal

BMC cancer
ISSN: 1471-2407
Titre abrégé: BMC Cancer
Pays: England
ID NLM: 100967800

Informations de publication

Date de publication:
13 Jun 2019
Historique:
received: 23 08 2018
accepted: 31 05 2019
entrez: 15 6 2019
pubmed: 15 6 2019
medline: 24 12 2019
Statut: epublish

Résumé

Treatment options in metastatic breast cancer are limited. New therapies preferable with predictive biomarkers are needed. The aim of these trials was to investigate if gene copy number of the topoisomerase 1 gene was predictive of response to the topoisomerase inhibitor irinotecan. Two open-label, single-arm phase II studies including HER2 positive and negative patients were conducted. Patients were eligible for inclusion if the primary tumor or a metastatic lesion had increased expression of the topoisomerase 1 gene defined as a TOP1 gene copy number of ≥4 or a TOP1/CEN20 ratio of ≥2. Patients were treated with irinotecan +/- trastuzumab weekly for 4 weeks following 2 weeks break, until progression or unacceptable toxicities. Evaluation scans were performed every 6 weeks. Primary endpoint was clinical benefit rate defined as the fraction of patients with stable disease for ≥4 months. The pre-planned number of 18 patients in each trial was not reached, thus no formal statistical analysis could be performed. Nine patients with HER2 negative disease and three patients with HER2 positive disease were included. Three patients obtained a partial remission and two patients had SD. The trials did not include the planned number of patients. No association between gene copy number of the topoisomerase 1 gene and response to irinotecan could be proved, however a clinical benefit was found in 5/12 patients and in 2/3 patients with HER2 positive disease. This could call for further investigation of the drug in the metastatic setting, especially in HER2 positive BC. Eudract registration numbers 2012-002348-26 and 2012-002347-23 . Registration date August 20th 2012.

Sections du résumé

BACKGROUND BACKGROUND
Treatment options in metastatic breast cancer are limited. New therapies preferable with predictive biomarkers are needed. The aim of these trials was to investigate if gene copy number of the topoisomerase 1 gene was predictive of response to the topoisomerase inhibitor irinotecan.
METHODS METHODS
Two open-label, single-arm phase II studies including HER2 positive and negative patients were conducted. Patients were eligible for inclusion if the primary tumor or a metastatic lesion had increased expression of the topoisomerase 1 gene defined as a TOP1 gene copy number of ≥4 or a TOP1/CEN20 ratio of ≥2. Patients were treated with irinotecan +/- trastuzumab weekly for 4 weeks following 2 weeks break, until progression or unacceptable toxicities. Evaluation scans were performed every 6 weeks. Primary endpoint was clinical benefit rate defined as the fraction of patients with stable disease for ≥4 months.
RESULTS RESULTS
The pre-planned number of 18 patients in each trial was not reached, thus no formal statistical analysis could be performed. Nine patients with HER2 negative disease and three patients with HER2 positive disease were included. Three patients obtained a partial remission and two patients had SD.
CONCLUSIONS CONCLUSIONS
The trials did not include the planned number of patients. No association between gene copy number of the topoisomerase 1 gene and response to irinotecan could be proved, however a clinical benefit was found in 5/12 patients and in 2/3 patients with HER2 positive disease. This could call for further investigation of the drug in the metastatic setting, especially in HER2 positive BC.
TRIAL REGISTRATION BACKGROUND
Eudract registration numbers 2012-002348-26 and 2012-002347-23 . Registration date August 20th 2012.

Identifiants

pubmed: 31196001
doi: 10.1186/s12885-019-5788-9
pii: 10.1186/s12885-019-5788-9
pmc: PMC6567440
doi:

Substances chimiques

Antineoplastic Agents 0
Biomarkers, Pharmacological 0
Topoisomerase I Inhibitors 0
Irinotecan 7673326042
ERBB2 protein, human EC 2.7.10.1
Receptor, ErbB-2 EC 2.7.10.1
DNA Topoisomerases, Type I EC 5.99.1.2
Trastuzumab P188ANX8CK

Types de publication

Clinical Trial, Phase II Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

573

Subventions

Organisme : Kræftens Bekæmpelse
ID : R56-Rp6818

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Auteurs

Iben Kümler (I)

Department of Oncology, Herlev and Gentofte Hospital, Herlev Ringvej 75, DK-2730, Herlev, Denmark. ibekml01@regionh.dk.

Eva Balslev (E)

Department of Pathology, Herlev and Gentofte Hospital, Herlev Ringvej 75, DK-2730, Herlev, Denmark.

Jan Stenvang (J)

Institut for Lægemiddeldesign og Farmakologi, Jagtvej 160, 2100, København Ø, Denmark.

Nils Brünner (N)

Institut for Lægemiddeldesign og Farmakologi, Jagtvej 160, 2100, København Ø, Denmark.

Bent Ejlertsen (B)

Department of Oncology, Rigshospitalet, Blegdamsvej 9, DK-2100, Copenhagen, Denmark.

Erik Hugger Jakobsen (EH)

Department of Oncology, Vejle Sygehus, Beriderbakken 4, DK-7100, Vejle, Denmark.

Dorte Lisbet Nielsen (DL)

Department of Oncology, Herlev and Gentofte Hospital, Herlev Ringvej 75, DK-2730, Herlev, Denmark.

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Classifications MeSH