Coherent diffractive imaging of microtubules using an X-ray laser.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
13 06 2019
13 06 2019
Historique:
received:
20
06
2018
accepted:
02
05
2019
entrez:
15
6
2019
pubmed:
15
6
2019
medline:
10
7
2019
Statut:
epublish
Résumé
X-ray free electron lasers (XFELs) create new possibilities for structural studies of biological objects that extend beyond what is possible with synchrotron radiation. Serial femtosecond crystallography has allowed high-resolution structures to be determined from micro-meter sized crystals, whereas single particle coherent X-ray imaging requires development to extend the resolution beyond a few tens of nanometers. Here we describe an intermediate approach: the XFEL imaging of biological assemblies with helical symmetry. We collected X-ray scattering images from samples of microtubules injected across an XFEL beam using a liquid microjet, sorted these images into class averages, merged these data into a diffraction pattern extending to 2 nm resolution, and reconstructed these data into a projection image of the microtubule. Details such as the 4 nm tubulin monomer became visible in this reconstruction. These results illustrate the potential of single-molecule X-ray imaging of biological assembles with helical symmetry at room temperature.
Identifiants
pubmed: 31197138
doi: 10.1038/s41467-019-10448-x
pii: 10.1038/s41467-019-10448-x
pmc: PMC6565740
doi:
Substances chimiques
Tubulin
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
2589Subventions
Organisme : Knut och Alice Wallenbergs Stiftelse (Knut and Alice Wallenberg Foundation)
ID : KAW 2012.0284
Pays : International
Organisme : NIGMS NIH HHS
ID : R01 GM109019
Pays : United States
Organisme : Vetenskapsrådet (Swedish Research Council)
ID : 2015-00560
Pays : International
Organisme : Knut och Alice Wallenbergs Stiftelse (Knut and Alice Wallenberg Foundation)
ID : KAW 2014.0275
Pays : International
Organisme : NCRR NIH HHS
ID : P41 RR001209
Pays : United States
Organisme : Knut och Alice Wallenbergs Stiftelse (Knut and Alice Wallenberg Foundation)
ID : KAW 2012.0275
Pays : International
Organisme : NIGMS NIH HHS
ID : P41 GM103393
Pays : United States
Commentaires et corrections
Type : ErratumIn
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