Immunogold FIB-SEM: Combining Volumetric Ultrastructure Visualization with 3D Biomolecular Analysis to Dissect Cell-Environment Interactions.

Cell Differentiation Dimethylpolysiloxanes Epigenesis, Genetic Humans Hydrogels Imaging, Three-Dimensional Microscopy, Electron, Scanning / methods Myoblasts / cytology Neural Stem Cells / ultrastructure Nuclear Pore / ultrastructure
3D reconstruction FIB-SEM electron microscopy image analysis immunogold

Journal

Advanced materials (Deerfield Beach, Fla.)
ISSN: 1521-4095
Titre abrégé: Adv Mater
Pays: Germany
ID NLM: 9885358

Informations de publication

Date de publication:
Aug 2019
Historique:
received: 21 01 2019
revised: 20 04 2019
pubmed: 15 6 2019
medline: 19 10 2019
entrez: 15 6 2019
Statut: ppublish

Résumé

Volumetric imaging techniques capable of correlating structural and functional information with nanoscale resolution are necessary to broaden the insight into cellular processes within complex biological systems. The recent emergence of focused ion beam scanning electron microscopy (FIB-SEM) has provided unparalleled insight through the volumetric investigation of ultrastructure; however, it does not provide biomolecular information at equivalent resolution. Here, immunogold FIB-SEM, which combines antigen labeling with in situ FIB-SEM imaging, is developed in order to spatially map ultrastructural and biomolecular information simultaneously. This method is applied to investigate two different cell-material systems: the localization of histone epigenetic modifications in neural stem cells cultured on microstructured substrates and the distribution of nuclear pore complexes in myoblasts differentiated on a soft hydrogel surface. Immunogold FIB-SEM offers the potential for broad applicability to correlate structure and function with nanoscale resolution when addressing questions across cell biology, biomaterials, and regenerative medicine.

Identifiants

DOI: 10.1002/adma.201900488 PMID: 31197896 PMC: PMC6778054
pubmed: 31197896
doi: 10.1002/adma.201900488
pmc: PMC6778054
mid: EMS83593
doi:

Substances chimiques

Dimethylpolysiloxanes 0
Hydrogels 0
baysilon 63148-62-9

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e1900488

Subventions

Organisme : Arthritis Research U.K. Foundation
ID : 21138
Organisme : Medical Research Council
ID : MR/R015651/1
Pays : United Kingdom
Organisme : European Research Council
ID : 616417
Pays : International
Organisme : ERC Seventh Framework Programme Consolidator
ID : 616417
Organisme : Medical Research Council
ID : MR/S00551X/1
Pays : United Kingdom
Organisme : Arthritis Research UK
ID : 21138
Pays : United Kingdom
Organisme : Wellcome Trust Senior Investigator Award
ID : 098411/Z/12/Z
Organisme : Wellcome Trust
ID : 098411
Pays : United Kingdom
Organisme : Versus Arthritis
ID : 21138
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_PC_16048
Pays : United Kingdom
Organisme : National Centre for the Replacement, Refinement and Reduction of Animals in Research
ID : NC/R001006/1
Pays : United Kingdom
Organisme : Australian Research Council
ID : IC160100026
Organisme : European Commission
ID : PIIF-GA-2011-302638 ERC StG 759577
Organisme : Australian Research Council
ID : DP110103543
Organisme : Royal Society
ID : NF110513
Organisme : Australian Research Council
ID : FT110100166
Organisme : Wellcome Trust
ID : 098411/Z/12/Z
Pays : United Kingdom
Organisme : Seventh Framework Programme
ID : 616417

Informations de copyright

© 2019 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Références

Biomaterials. 2011 Aug;32(24):5568-80
pubmed: 21570113
Nat Neurosci. 2013 Jul;16(7):790-7
pubmed: 23799471
J Microsc. 2014 Jun;254(3):109-14
pubmed: 24707797
Tissue Eng Part A. 2017 May;23(9-10):426-435
pubmed: 28103756
ACS Nano. 2015 May 26;9(5):5500-5509
pubmed: 25858596
Biomaterials. 2015 Nov;70:94-104
pubmed: 26302234
J Neurosci. 2009 Nov 25;29(47):14687-700
pubmed: 19940164
Nucleus. 2017 Sep 3;8(5):506-514
pubmed: 28686487
Nat Mater. 2013 Dec;12(12):1154-62
pubmed: 24141451
Genome Biol. 2015 Aug 25;16:174
pubmed: 26303512
J Cell Sci. 2011 Dec 15;124(Pt 24):4253-66
pubmed: 22223883
Nat Methods. 2012 Jan 30;9(2):152-8
pubmed: 22290187
Cell. 2006 Aug 25;126(4):677-89
pubmed: 16923388
Microsyst Nanoeng. 2017 Mar 13;3:16080
pubmed: 31057850
Acta Biomater. 2006 Jul;2(4):409-19
pubmed: 16765881
J Biomed Mater Res A. 2008 Mar 15;84(4):973-9
pubmed: 17647239
J Neurochem. 2015 Nov;135(4):643-58
pubmed: 26222552
Dev Cell. 2008 Aug;15(2):182-6
pubmed: 18694558
Trends Genet. 2016 Jan;32(1):29-41
pubmed: 26675384
ACS Nano. 2017 Aug 22;11(8):8320-8328
pubmed: 28682058
Trends Cell Biol. 2011 Jun;21(6):362-73
pubmed: 21514163
Am J Physiol Heart Circ Physiol. 2005 Apr;288(4):H1915-24
pubmed: 15576436
Exp Cell Res. 2015 Oct 1;337(2):202-7
pubmed: 26272543
Small. 2013 Dec 9;9(23):4006-16, 3905
pubmed: 23813871
J Cell Biol. 2010 Jul 26;190(2):165-75
pubmed: 20643879
J Cell Sci. 2014 Jan 1;127(Pt 1):124-36
pubmed: 24144701
Methods. 1998 Oct;16(2):170-81
pubmed: 9790863

Auteurs

Sahana Gopal (S)

Department of Materials, Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, UK.
Department of Medicine, Imperial College London, London, W12 0NN, UK.

Ciro Chiappini (C)

Department of Materials, Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, UK.
Centre for Craniofacial and Regenerative Biology, King's College London, London, SE1 9RT, UK.

James P K Armstrong (JPK)

Department of Materials, Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, UK.

Qu Chen (Q)

Department of Materials, Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, UK.

Andrea Serio (A)

Department of Materials, Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, UK.
Centre for Craniofacial and Regenerative Biology, King's College London, London, SE1 9RT, UK.

Chia-Chen Hsu (CC)

Department of Materials, Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, UK.
ORCID: 0000-0003-4408-8297

Christoph Meinert (C)

Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, 4059, Australia.

Travis J Klein (TJ)

Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, 4059, Australia.
Australian Research Council Industrial Transformation Training Centre, Queensland University of Technology, Brisbane, Queensland, 4059, Australia.

Dietmar W Hutmacher (DW)

Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, 4059, Australia.
Australian Research Council Industrial Transformation Training Centre, Queensland University of Technology, Brisbane, Queensland, 4059, Australia.

Stephen Rothery (S)

Facility for Light Microscopy, Imperial College London, London, SW7 2AZ, UK.

Molly M Stevens (MM)

Department of Materials, Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, UK.
ORCID: 0000-0002-6688-5126

Articles similaires

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female

Evaluation of Low-Value Services Across Major Medicare Advantage Insurers and Traditional Medicare.

Ciara Duggan, Adam L Beckman, Ishani Ganguli et al.
1.00
Humans United States Aged Cross-Sectional Studies Medicare Part C

Effectiveness of Virtual Yoga for Chronic Low Back Pain: A Randomized Clinical Trial.

Hallie Tankha, Devyn Gaskins, Amanda Shallcross et al.
1.00
Humans Yoga Low Back Pain Female Male

Classifications MeSH

questionsmedicales.fr › Phénomènes physiologiques cellulaires › Différenciation cellulaire › Immunogold FIB-SEM: Combining Volumetric...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Polymères › Siloxanes › Silicone › Polydiméthylsiloxanes › Immunogold FIB-SEM: Combining Volumetric...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Immunogold FIB-SEM: Combining Volumetric...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Immunogold FIB-SEM: Combining Volumetric...
questionsmedicales.fr › Préparations pharmaceutiques › Colloïdes › Gels › Hydrogels › Immunogold FIB-SEM: Combining Volumetric...
questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Traitement d'image par ordinateur › Imagerie tridimensionnelle › Immunogold FIB-SEM: Combining Volumetric...
questionsmedicales.fr › Techniques d'investigation › Microscopie électronique › Microscopie électronique à balayage › Immunogold FIB-SEM: Combining Volumetric...
questionsmedicales.fr › Cellules › Cellules souches › Myoblastes › Immunogold FIB-SEM: Combining Volumetric...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches neurales › Immunogold FIB-SEM: Combining Volumetric...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Structures nucléaires › Pore nucléaire › Immunogold FIB-SEM: Combining Volumetric...