Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter.
Air Pollutants
/ toxicity
Animals
Biomarkers
/ chemistry
Chromatography, High Pressure Liquid
/ methods
DNA
/ chemistry
DNA Adducts
DNA Damage
Deoxyadenosines
/ chemistry
Deoxyguanosine
/ chemistry
Male
Mice
Mutagens
/ toxicity
Oxidation-Reduction
Particulate Matter
/ analysis
Tandem Mass Spectrometry
/ methods
Journal
Journal of visualized experiments : JoVE
ISSN: 1940-087X
Titre abrégé: J Vis Exp
Pays: United States
ID NLM: 101313252
Informations de publication
Date de publication:
29 05 2019
29 05 2019
Historique:
entrez:
18
6
2019
pubmed:
18
6
2019
medline:
2
4
2020
Statut:
epublish
Résumé
DNA adducts and oxidized DNA bases are examples of DNA lesions that are useful biomarkers for the toxicity assessment of substances that are electrophilic, generate reactive electrophiles upon biotransformation, or induce oxidative stress. Among the oxidized nucleobases, the most studied one is 8-oxo-7,8-dihydroguanine (8-oxoGua) or 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo), a biomarker of oxidatively induced base damage in DNA. Aldehydes and epoxyaldehydes resulting from the lipid peroxidation process are electrophilic molecules able to form mutagenic exocyclic DNA adducts, such as the etheno adducts 1,N
Substances chimiques
Air Pollutants
0
Biomarkers
0
DNA Adducts
0
Deoxyadenosines
0
Mutagens
0
Particulate Matter
0
DNA
9007-49-2
Deoxyguanosine
G9481N71RO
2'-deoxyadenosine
P582C98ULC
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Validation Study
Video-Audio Media
Langues
eng
Sous-ensembles de citation
IM