Conformational switches control early maturation of the eukaryotic small ribosomal subunit.
Cryoelectron Microscopy
Eukaryotic Cells
/ metabolism
Models, Molecular
Molecular Conformation
Protein Domains
RNA Precursors
/ chemistry
RNA, Ribosomal, 18S
/ chemistry
Ribosomal Proteins
/ chemistry
Ribosome Subunits, Small
/ chemistry
Ribosome Subunits, Small, Eukaryotic
/ chemistry
Saccharomyces cerevisiae Proteins
/ chemistry
RNA
S. cerevisiae
biochemistry
chemical biology
cryo-EM
molecular biophysics
protein complexes
ribosome biogenesis
structural biology
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
17 06 2019
17 06 2019
Historique:
received:
14
01
2019
accepted:
05
06
2019
entrez:
18
6
2019
pubmed:
18
6
2019
medline:
26
2
2020
Statut:
epublish
Résumé
Eukaryotic ribosome biogenesis is initiated with the transcription of pre-ribosomal RNA at the 5' external transcribed spacer, which directs the early association of assembly factors but is absent from the mature ribosome. The subsequent co-transcriptional association of ribosome assembly factors with pre-ribosomal RNA results in the formation of the small subunit processome. Here we show that stable rRNA domains of the small ribosomal subunit can independently recruit their own biogenesis factors in vivo. The final assembly and compaction of the small subunit processome requires the presence of the 5' external transcribed spacer RNA and all ribosomal RNA domains. Additionally, our cryo-electron microscopy structure of the earliest nucleolar pre-ribosomal assembly - the 5' external transcribed spacer ribonucleoprotein - provides a mechanism for how conformational changes in multi-protein complexes can be employed to regulate the accessibility of binding sites and therefore define the chronology of maturation events during early stages of ribosome assembly.
Identifiants
pubmed: 31206356
doi: 10.7554/eLife.45185
pii: 45185
pmc: PMC6579516
doi:
pii:
Substances chimiques
RNA Precursors
0
RNA, Ribosomal, 18S
0
Ribosomal Proteins
0
Saccharomyces cerevisiae Proteins
0
Banques de données
PDB
['6ND4']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : DP2 GM123459
Pays : United States
Organisme : Swiss National Science Foundation
ID : 155515
Pays : Switzerland
Organisme : NIH HHS
ID : 1DP2GM123459
Pays : United States
Informations de copyright
© 2019, Hunziker et al.
Déclaration de conflit d'intérêts
MH, JB, OB, CS, HM, SK No competing interests declared
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