Unique Regulation of Na-K-ATPase during Growth and Maturation of Intestinal Epithelial Cells.
Alkaline Phosphatase
/ metabolism
Animals
Cell Differentiation
Cell Line
Cell Proliferation
Epithelial Cells
/ cytology
Glucose
/ metabolism
Intestines
/ cytology
Kinetics
Phosphorylation
Protein Subunits
/ genetics
RNA, Messenger
/ genetics
Rats
Sodium
/ metabolism
Sodium-Potassium-Exchanging ATPase
/ genetics
Na-dependent nutrient co-transport
Na/K-ATPase
cell maturation
crypt cells
intestinal absorption
villus cells
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
15 06 2019
15 06 2019
Historique:
received:
25
05
2019
revised:
09
06
2019
accepted:
13
06
2019
entrez:
19
6
2019
pubmed:
19
6
2019
medline:
19
6
2019
Statut:
epublish
Résumé
Na-K-ATPase on the basolateral membrane provides the favorable transcellular Na gradient for the proper functioning of Na-dependent nutrient co-transporters on the brush border membrane (BBM) of enterocytes. As cells mature from crypts to villus, Na-K-ATPase activity doubles, to accommodate for the increased BBM Na-dependent nutrient absorption. However, the mechanism of increased Na-K-ATPase activity during the maturation of enterocytes is not known. Therefore, this study aimed to determine the mechanisms involved in the functional transition of Na-K-ATPase during the maturation of crypts to villus cells. Na-K-ATPase activity gradually increased as IEC-18 cells matured in vitro from day 0 (crypts) through day 4 (villus) of post-confluence. mRNA abundance and Western blot studies showed no change in the levels of Na-K-ATPase subunits α1 and β1 from 0 to 4 days post-confluent cells. However, Na-K-ATPase α1 phosphorylation levels on serine and tyrosine, but not threonine, residues gradually increased. These data indicate that as enterocytes mature from crypt-like to villus-like in culture, the functional activity of Na-K-ATPase increases secondary to altered affinity of the α1 subunit to extracellular K
Identifiants
pubmed: 31208048
pii: cells8060593
doi: 10.3390/cells8060593
pmc: PMC6628168
pii:
doi:
Substances chimiques
Protein Subunits
0
RNA, Messenger
0
Sodium
9NEZ333N27
Alkaline Phosphatase
EC 3.1.3.1
Sodium-Potassium-Exchanging ATPase
EC 7.2.2.13
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : P20 GM121299
Pays : United States
Références
Am J Physiol. 1993 Dec;265(6 Pt 2):F743-55
pubmed: 8285207
J Perinat Med. 1978;6(3):154-9
pubmed: 212544
J Membr Biol. 2010 Feb;233(1-3):119-26
pubmed: 20130847
Am J Physiol. 1995 Aug;269(2 Pt 1):C295-311
pubmed: 7653511
J Biol Chem. 2004 Jun 11;279(24):25211-8
pubmed: 15069082
Semin Nephrol. 2005 Sep;25(5):328-34
pubmed: 16139688
Scand J Gastroenterol. 1989 Nov;24(9):1121-5
pubmed: 2556782
J Biol Chem. 1994 Sep 30;269(39):24437-45
pubmed: 7929106
Hear Res. 1996 Oct;100(1-2):143-9
pubmed: 8922988
J Bioenerg Biomembr. 2001 Oct;33(5):439-47
pubmed: 11762919
Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11359-62
pubmed: 1662394
Biochem Biophys Res Commun. 1991 Dec 31;181(3):1208-17
pubmed: 1764071
Am J Physiol Cell Physiol. 2000 Nov;279(5):C1621-30
pubmed: 11029310
J Cell Physiol. 1993 Feb;154(2):271-80
pubmed: 8381125
Sci Rep. 2016 Aug 25;6:31917
pubmed: 27558220
Nature. 1985 Aug 22-28;316(6030):691-5
pubmed: 2993903
FEBS Lett. 1990 Aug 1;268(2):314-24
pubmed: 2166689
J Biol Chem. 1994 Mar 25;269(12):9368-73
pubmed: 7510709
Acta Physiol Scand Suppl. 1992;607:161-9
pubmed: 1333149
Pediatr Res. 1993 Jan;33(1):1-4
pubmed: 8381951
Am J Physiol. 1994 Aug;267(2 Pt 1):C450-5
pubmed: 8074180
Biochim Biophys Acta. 2015 Feb;1848(2):702-11
pubmed: 25462166
J Biol Chem. 1992 Nov 5;267(31):22378-84
pubmed: 1331053
Mol Biol Cell. 1999 Sep;10(9):2847-59
pubmed: 10473631
Curr Opin Nephrol Hypertens. 1993 Sep;2(5):725-34
pubmed: 7922215
BMC Gastroenterol. 2015 Apr 15;15:47
pubmed: 25884559
Nature. 1986 May 22-28;321(6068):429-31
pubmed: 3012356
J Bioenerg Biomembr. 2001 Oct;33(5):425-38
pubmed: 11762918
Comp Biochem Physiol A Mol Integr Physiol. 2018 Nov;225:16-25
pubmed: 29932975
FEBS Lett. 1990 Aug 20;269(1):105-8
pubmed: 2167238
Biochim Biophys Acta. 1981 Dec 21;649(3):625-32
pubmed: 7317421
J Biol Chem. 2012 Jul 27;287(31):26115-25
pubmed: 22696220
Anal Biochem. 1983 Jan;128(1):159-63
pubmed: 6303151
Arch Biochem Biophys. 1998 Nov 15;359(2):139-50
pubmed: 9808755
Eur Arch Otorhinolaryngol. 1993;250(5):265-70
pubmed: 8217127
Can J Biochem Cell Biol. 1983 Jan;61(1):23-8
pubmed: 6303531
FASEB J. 1990 Apr 1;4(6):1598-605
pubmed: 2156741
Annu Rev Biochem. 2002;71:511-35
pubmed: 12045105
J Biol Chem. 1999 Nov 19;274(47):33183-5
pubmed: 10559186
Am J Physiol. 1997 Apr;272(4 Pt 1):G732-41
pubmed: 9142903
Front Cell Dev Biol. 2017 May 08;5:41
pubmed: 28534026
J Anim Sci. 2001 Feb;79(2):371-81
pubmed: 11219446
J Biol Chem. 2010 Nov 19;285(47):36330-8
pubmed: 20841356
J Bioenerg Biomembr. 1992 Jun;24(3):249-61
pubmed: 1328174
Isr J Med Sci. 1984 Aug;20(8):681-4
pubmed: 6088428
Biochemistry. 1999 Oct 12;38(41):13661-9
pubmed: 10521273
Arch Dis Child Fetal Neonatal Ed. 2000 Sep;83(2):F135-8
pubmed: 10952709
EMBO J. 1997 Jul 16;16(14):4250-60
pubmed: 9250668
Cell Mol Life Sci. 2012 Sep;69(17):2907-17
pubmed: 22527717
Cell Mol Life Sci. 2003 Jul;60(7):1322-32
pubmed: 12943221
Am J Physiol Gastrointest Liver Physiol. 2005 Dec;289(6):G1030-5
pubmed: 16099871
Am J Physiol. 1998 Nov;275(5):F633-50
pubmed: 9815123
Int Rev Cytol. 1993;137C:139-68
pubmed: 8387469
Nature. 1978 Apr 27;272(5656):827-9
pubmed: 205798
J Membr Biol. 2010 Mar;234(1):35-45
pubmed: 20177668