Live calcium imaging of Aedes aegypti neuronal tissues reveals differential importance of chemosensory systems for life-history-specific foraging strategies.
Aedes aegypti
Calcium
GCaMP6s
GECI
Neuronal
Stimuli-evoked responses
Journal
BMC neuroscience
ISSN: 1471-2202
Titre abrégé: BMC Neurosci
Pays: England
ID NLM: 100966986
Informations de publication
Date de publication:
17 06 2019
17 06 2019
Historique:
received:
24
01
2019
accepted:
10
06
2019
entrez:
19
6
2019
pubmed:
19
6
2019
medline:
29
1
2020
Statut:
epublish
Résumé
The mosquito Aedes aegypti has a wide variety of sensory pathways that have supported its success as a species as well as a highly competent vector of numerous debilitating infectious pathogens. Investigations into mosquito sensory systems and their effects on behavior are valuable resources for the advancement of mosquito control strategies. Numerous studies have elucidated key aspects of mosquito sensory systems, however there remains critical gaps within the field. In particular, compared to that of the adult form, there has been a lack of studies directed towards the immature life stages. Additionally, although numerous studies have pinpointed specific sensory receptors as well as responding motor outputs, there has been a lack of studies able to monitor both concurrently. To begin filling aforementioned gaps, here we engineered Ae. aegypti to ubiquitously express a genetically encoded calcium indicator, GCaMP6s. Using this strain, combined with advanced microscopy, we simultaneously measured live stimulus-evoked calcium responses in both neuronal and muscle cells with a wide spatial range and resolution. By coupling in vivo live calcium imaging with behavioral assays we were able to gain functional insights into how stimulus-evoked neural and muscle activities are represented, modulated, and transformed in mosquito larvae enabling us to elucidate mosquito sensorimotor properties important for life-history-specific foraging strategies.
Sections du résumé
BACKGROUND
The mosquito Aedes aegypti has a wide variety of sensory pathways that have supported its success as a species as well as a highly competent vector of numerous debilitating infectious pathogens. Investigations into mosquito sensory systems and their effects on behavior are valuable resources for the advancement of mosquito control strategies. Numerous studies have elucidated key aspects of mosquito sensory systems, however there remains critical gaps within the field. In particular, compared to that of the adult form, there has been a lack of studies directed towards the immature life stages. Additionally, although numerous studies have pinpointed specific sensory receptors as well as responding motor outputs, there has been a lack of studies able to monitor both concurrently.
RESULTS
To begin filling aforementioned gaps, here we engineered Ae. aegypti to ubiquitously express a genetically encoded calcium indicator, GCaMP6s. Using this strain, combined with advanced microscopy, we simultaneously measured live stimulus-evoked calcium responses in both neuronal and muscle cells with a wide spatial range and resolution.
CONCLUSIONS
By coupling in vivo live calcium imaging with behavioral assays we were able to gain functional insights into how stimulus-evoked neural and muscle activities are represented, modulated, and transformed in mosquito larvae enabling us to elucidate mosquito sensorimotor properties important for life-history-specific foraging strategies.
Identifiants
pubmed: 31208328
doi: 10.1186/s12868-019-0511-y
pii: 10.1186/s12868-019-0511-y
pmc: PMC6580577
doi:
Substances chimiques
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
27Subventions
Organisme : NIAID NIH HHS
ID : R21 AI123937
Pays : United States
Organisme : NIDCD NIH HHS
ID : R01 DC013693
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI123937
Pays : United States
Références
Nat Commun. 2018 Oct 22;9(1):4390
pubmed: 30348941
Nature. 2013 Jul 18;499(7458):295-300
pubmed: 23868258
Science. 2009 Jan 2;323(5910):141-4
pubmed: 19119237
Insect Mol Biol. 2012 Feb;21(1):119-27
pubmed: 22122783
Nature. 2011 Aug 24;476(7361):450-3
pubmed: 21866159
Curr Biol. 2017 Apr 24;27(8):1200-1205
pubmed: 28392112
Elife. 2020 Jan 21;9:
pubmed: 31960794
Curr Biol. 2002 Apr 30;12(9):730-4
pubmed: 12007416
Nat Protoc. 2011 Jan;6(1):28-35
pubmed: 21212780
Curr Biol. 2018 Feb 5;28(3):333-344.e8
pubmed: 29395917
J Am Mosq Control Assoc. 1989 Sep;5(3):311-6
pubmed: 2573687
Trends Plant Sci. 2004 May;9(5):253-61
pubmed: 15130551
Appl Environ Microbiol. 2004 May;70(5):2823-9
pubmed: 15128538
Curr Trop Med Rep. 2014 Mar 1;1(1):21-31
pubmed: 24693489
Curr Opin Cell Biol. 2012 Apr;24(2):262-8
pubmed: 22169400
Annu Rev Med. 2018 Jan 29;69:395-408
pubmed: 28846489
Science. 1968 Sep 27;161(3848):1346-7
pubmed: 5673445
Annu Rev Entomol. 2008;53:273-91
pubmed: 17803458
Proc Natl Acad Sci U S A. 2014 Jan 28;111(4):1598-603
pubmed: 24474785
Nat Biotechnol. 2012 Sep;30(9):828-30
pubmed: 22965050
ACS Chem Biol. 2018 Feb 16;13(2):424-430
pubmed: 29370514
J Insect Physiol. 2012 Apr;58(4):506-12
pubmed: 22172381
J Am Mosq Control Assoc. 2007 Mar;23(1):11-7
pubmed: 17536362
Arch Microbiol. 2003 Jan-Feb;179(2):75-82
pubmed: 12560984
Insect Biochem Mol Biol. 2001 Nov 1;31(12):1137-43
pubmed: 11583926
Nat Commun. 2019 Jan 8;10(1):84
pubmed: 30622266
Annu Rev Entomol. 1992;37:349-76
pubmed: 1347208
Insect Mol Biol. 2010 Aug;19(4):441-9
pubmed: 20456509
Virology. 1992 Mar;187(1):97-106
pubmed: 1736547
J Chem Ecol. 2008 Jul;34(7):837-53
pubmed: 18548311
Nat Rev Microbiol. 2004 Oct;2(10):789-801
pubmed: 15378043
Dev Genes Evol. 2011 Dec;221(5-6):281-96
pubmed: 21956584
Curr Opin Insect Sci. 2015 Aug;10:83-89
pubmed: 29588018
PLoS Negl Trop Dis. 2017 Jul 20;11(7):e0005625
pubmed: 28727779
Curr Opin Insect Sci. 2017 Apr;20:75-83
pubmed: 28602240
Proc Natl Acad Sci U S A. 2008 Apr 29;105(17):6433-8
pubmed: 18427108
PLoS Negl Trop Dis. 2017 Jul 17;11(7):e0005651
pubmed: 28715426
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Antiviral Res. 2010 Feb;85(2):328-45
pubmed: 19857523
J Insect Sci. 2011;11:62
pubmed: 21864156
Annu Rev Entomol. 2007;52:209-29
pubmed: 16913829
Proc Natl Acad Sci U S A. 2017 Dec 5;114(49):E10540-E10549
pubmed: 29138316
Insect Mol Biol. 1999 Nov;8(4):449-57
pubmed: 10634970
PLoS Biol. 2010 Aug 31;8(8):
pubmed: 20824161
Nat Commun. 2016 Oct 03;7:13010
pubmed: 27694947
J Exp Biol. 2008 Sep;211(Pt 18):3020-7
pubmed: 18775939
Insect Mol Biol. 2007 Oct;16(5):525-37
pubmed: 17635615
Curr Biol. 2015 Aug 31;25(17):2203-14
pubmed: 26299514
Nat Commun. 2011 Aug 23;2:441
pubmed: 21863008
Curr Biol. 2017 Feb 6;27(3):345-358
pubmed: 28132816
Nat Rev Genet. 2016 Mar;17(3):146-59
pubmed: 26875679
ACS Chem Biol. 2012 Oct 19;7(10):1647-52
pubmed: 22924767
Curr Biol. 2013 Jul 8;23(13):1228-34
pubmed: 23770186
Nature. 2013 Jun 27;498(7455):487-91
pubmed: 23719379
PLoS One. 2009 Sep 15;4(9):e7032
pubmed: 19753115
Biol Bull. 2007 Aug;213(1):1-11
pubmed: 17679714
Elife. 2018 Jun 26;7:
pubmed: 29943730
J Chem Ecol. 2010 Mar;36(3):245-8
pubmed: 20191395
Proc Natl Acad Sci U S A. 2012 Apr 24;109(17):6626-31
pubmed: 22493255
Cell. 2017 Jan 12;168(1-2):280-294.e12
pubmed: 28065412
Insect Mol Biol. 2002 Apr;11(2):133-9
pubmed: 11966878
PLoS One. 2013 Jul 23;8(7):e70218
pubmed: 23894621
Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3312-7
pubmed: 17360644
PLoS Negl Trop Dis. 2013 May 16;7(5):e2215
pubmed: 23696908
Insect Biochem Mol Biol. 2004 Jul;34(7):723-9
pubmed: 15242714
Cell. 2014 Feb 27;156(5):1060-71
pubmed: 24581501
Curr Biol. 2015 Aug 17;25(16):2123-9
pubmed: 26190071
Curr Opin Neurobiol. 2009 Aug;19(4):345-53
pubmed: 19660932
J Neurophysiol. 2002 Nov;88(5):2659-63
pubmed: 12424301
Methods. 2014 Aug 15;69(1):38-45
pubmed: 24556554
J Chem Ecol. 2002 Oct;28(10):1901-17
pubmed: 12474890
Nat Methods. 2009 May;6(5):343-5
pubmed: 19363495
J Med Entomol. 2015 Nov;52(6):1315-21
pubmed: 26352935
Cold Spring Harb Protoc. 2009 Apr;2009(4):pdb.prot5199
pubmed: 20147142
G3 (Bethesda). 2013 Sep 04;3(9):1493-509
pubmed: 23833213
Sci Rep. 2014 Feb 04;4:3954
pubmed: 24492376