Expression of Ki-67, p53, α-SMA and COX-2 in lichen planus and related lesions: A pilot study.

Epithelial dysplasia Immunohistochemistry Lichenoid reaction Oral lichen planus

Journal

Journal of oral biology and craniofacial research
ISSN: 2212-4268
Titre abrégé: J Oral Biol Craniofac Res
Pays: Netherlands
ID NLM: 101619156

Informations de publication

Date de publication:
Historique:
received: 19 08 2017
accepted: 18 02 2018
entrez: 19 6 2019
pubmed: 19 6 2019
medline: 19 6 2019
Statut: ppublish

Résumé

Researchers have struggled to understand the natural history of lesions presenting with both lichenoid features and epithelial dysplasia. Thus the present study was designed to differentiate between OLP, OLP with dysplasia, epithelial dysplasia and epithelial dysplasia with lichenoid features based on the expressions of ki-67, p53, COX-2, and α-SMA. Formalin-fixed paraffin-embedded archival specimens of OLP, OLP with dysplasia, epithelial dysplasia and epithelial dysplasia with lichenoid features were subjected to immunohistochemical staining with ki-67, p53, COX-2, and α-SMA. Ki-67 exhibited strong positivity in 100% (6/6) of epithelial dysplasia cases, 71.4% (5/7) of lichenoid dysplasia cases, 57.1% (4/7) of OLP cases and 60% (3/5) of OLP with dysplasia cases. Strong p53 staining was evident in more cases of lichenoid dysplasia [42.8% (3/7)], while moderate staining was more frequent in OLP cases [42.8% (3/7)] and OLP with dysplasia cases [42.8% (3/7)] and mild intensity was more frequent in epithelial dysplasia cases [50% (3/6)] followed by lichenoid dysplasia cases [42.8% (3/7)], OLP cases [28.5 (2/7)] and OLP with dysplasia cases [40% (2/5)]. COX-2 strong positivity was more frequent in cases of epithelial dysplasia cases [57.1% (4/7)] and OLP [50% (3/6)]. Strong α- SMA staining was noted more frequently in lichenoid dysplasia cases [71.4 (5/7)], followed by OLP cases [42.8% (3/7)] and OLP with dysplasia cases [60% (3/5)]. Ki-67, p53, α-SMA and COX-2 expression do not differentiate between OLP, LP with dysplasia and epithelial dysplasia with lichenoid features.

Sections du résumé

BACKGROUND BACKGROUND
Researchers have struggled to understand the natural history of lesions presenting with both lichenoid features and epithelial dysplasia. Thus the present study was designed to differentiate between OLP, OLP with dysplasia, epithelial dysplasia and epithelial dysplasia with lichenoid features based on the expressions of ki-67, p53, COX-2, and α-SMA.
MATERIALS AND METHODS METHODS
Formalin-fixed paraffin-embedded archival specimens of OLP, OLP with dysplasia, epithelial dysplasia and epithelial dysplasia with lichenoid features were subjected to immunohistochemical staining with ki-67, p53, COX-2, and α-SMA.
RESULTS RESULTS
Ki-67 exhibited strong positivity in 100% (6/6) of epithelial dysplasia cases, 71.4% (5/7) of lichenoid dysplasia cases, 57.1% (4/7) of OLP cases and 60% (3/5) of OLP with dysplasia cases. Strong p53 staining was evident in more cases of lichenoid dysplasia [42.8% (3/7)], while moderate staining was more frequent in OLP cases [42.8% (3/7)] and OLP with dysplasia cases [42.8% (3/7)] and mild intensity was more frequent in epithelial dysplasia cases [50% (3/6)] followed by lichenoid dysplasia cases [42.8% (3/7)], OLP cases [28.5 (2/7)] and OLP with dysplasia cases [40% (2/5)]. COX-2 strong positivity was more frequent in cases of epithelial dysplasia cases [57.1% (4/7)] and OLP [50% (3/6)]. Strong α- SMA staining was noted more frequently in lichenoid dysplasia cases [71.4 (5/7)], followed by OLP cases [42.8% (3/7)] and OLP with dysplasia cases [60% (3/5)].
CONCLUSIONS CONCLUSIONS
Ki-67, p53, α-SMA and COX-2 expression do not differentiate between OLP, LP with dysplasia and epithelial dysplasia with lichenoid features.

Identifiants

pubmed: 31211042
doi: 10.1016/j.jobcr.2018.02.003
pii: S2212-4268(17)30194-X
pmc: PMC6562184
doi:

Types de publication

Journal Article

Langues

eng

Pagination

230-235

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Auteurs

D S Sanketh (DS)

Department of Oral Pathology and Microbiology, Faculty of Dental Sciences, Ramaiah University of Applied sciences, Bangalore, India.

Karuna Kumari (K)

Department of Oral Pathology and Microbiology, Faculty of Dental Sciences, Ramaiah University of Applied sciences, Bangalore, India.

Roopa S Rao (RS)

Department of Oral Pathology and Microbiology, Faculty of Dental Sciences, Ramaiah University of Applied sciences, Bangalore, India.

Vanishree C Haragannavar (VC)

Department of Oral Pathology and Microbiology, Faculty of Dental Sciences, Ramaiah University of Applied sciences, Bangalore, India.

Sachin C Sarode (SC)

Department of Oral Pathology and Microbiology, Dr. D.Y. Patil Dental College and Hospital, Dr. D.Y. Patil Vidyapeeth, Sant-Tukaram Nagar, Pimpri, Pune 411018, India.

Gargi S Sarode (GS)

Department of Oral Pathology and Microbiology, Dr. D.Y. Patil Dental College and Hospital, Dr. D.Y. Patil Vidyapeeth, Sant-Tukaram Nagar, Pimpri, Pune 411018, India.

A Thirumal Raj (AT)

Department of Oral Pathology and Microbiology, Sri Venkateswara Dental College, and Hospital, Chennai, India.

Shankargouda Patil (S)

Department of Maxillofacial Surgery and Diagnostic Sciences, Division of Oral Pathology, College of Dentistry, Jazan University, Jazan, Saudi Arabia.

Classifications MeSH