Rapid Purification of Endotoxin-Free RTX Toxins.
Animals
Bacterial Proteins
/ isolation & purification
Cell Line, Tumor
Cell Survival
/ drug effects
Cytotoxins
/ isolation & purification
Detergents
/ chemistry
Erythrocytes
/ drug effects
Escherichia coli
/ metabolism
Hemolysin Proteins
/ isolation & purification
Hemolysis
Humans
Lipopolysaccharides
/ analysis
Octoxynol
/ chemistry
Sheep
THP-1 Cells
Urea
/ chemistry
RTX toxins
Triton X-100
Triton X-114
endotoxin
lipopolysaccharide
Journal
Toxins
ISSN: 2072-6651
Titre abrégé: Toxins (Basel)
Pays: Switzerland
ID NLM: 101530765
Informations de publication
Date de publication:
12 06 2019
12 06 2019
Historique:
received:
14
05
2019
revised:
05
06
2019
accepted:
07
06
2019
entrez:
20
6
2019
pubmed:
20
6
2019
medline:
17
3
2020
Statut:
epublish
Résumé
Cytolytic leukotoxins of the repeat in toxin (RTX) family are large proteins excreted by gram-negative bacterial pathogens through the type 1 secretion system (T1SS). Due to low yields and poor stability in cultures of the original pathogens, it is useful to purify recombinant fatty-acylated RTX cytolysins from inclusion bodies produced in
Identifiants
pubmed: 31212877
pii: toxins11060336
doi: 10.3390/toxins11060336
pmc: PMC6628407
pii:
doi:
Substances chimiques
Bacterial Proteins
0
Cytotoxins
0
Detergents
0
Hemolysin Proteins
0
Lipopolysaccharides
0
Urea
8W8T17847W
Octoxynol
9002-93-1
Nonidet P-40
9036-19-5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
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