Design and Use of Chikungunya Virus Replication Templates Utilizing Mammalian and Mosquito RNA Polymerase I-Mediated Transcription.


Journal

Journal of virology
ISSN: 1098-5514
Titre abrégé: J Virol
Pays: United States
ID NLM: 0113724

Informations de publication

Date de publication:
15 09 2019
Historique:
received: 10 05 2019
accepted: 18 06 2019
pubmed: 21 6 2019
medline: 8 7 2020
entrez: 21 6 2019
Statut: epublish

Résumé

Chikungunya virus (CHIKV) is a mosquito-borne alphavirus. It has a positive-sense RNA genome that also serves as the mRNA for four nonstructural proteins (nsPs) representing subunits of the viral replicase. Coupling of nsP and RNA synthesis complicates analysis of viral RNA replication. We developed

Identifiants

pubmed: 31217251
pii: JVI.00794-19
doi: 10.1128/JVI.00794-19
pmc: PMC6714806
pii:
doi:

Substances chimiques

Antiviral Agents 0
RNA, Viral 0
Viral Nonstructural Proteins 0
RNA Polymerase I EC 2.7.7.6

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_PC_16028
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 200171/Z/15/Z
Pays : United Kingdom

Informations de copyright

Copyright © 2019 American Society for Microbiology.

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Auteurs

Age Utt (A)

Institute of Technology, University of Tartu, Tartu, Estonia.

Kai Rausalu (K)

Institute of Technology, University of Tartu, Tartu, Estonia.

Madis Jakobson (M)

Icosagen AS, Õssu, Tartumaa, Estonia.

Andres Männik (A)

Institute of Technology, University of Tartu, Tartu, Estonia.
Icosagen AS, Õssu, Tartumaa, Estonia.

Luke Alphey (L)

The Pirbright Institute, Woking, United Kingdom.

Rennos Fragkoudis (R)

The Pirbright Institute, Woking, United Kingdom.
School of Veterinary Medicine and Science, University of Nottingham, Loughborough, United Kingdom.

Andres Merits (A)

Institute of Technology, University of Tartu, Tartu, Estonia andres.merits@ut.ee.

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Classifications MeSH