Rapid Detection of Senescent Mesenchymal Stromal Cells by a Fluorescent Probe.
aging
bioprocessing
fluorescent probe
mesenchymal stromal cells
senescence
stem cells
Journal
Biotechnology journal
ISSN: 1860-7314
Titre abrégé: Biotechnol J
Pays: Germany
ID NLM: 101265833
Informations de publication
Date de publication:
Oct 2019
Oct 2019
Historique:
received:
22
11
2018
revised:
10
06
2019
pubmed:
21
6
2019
medline:
11
2
2020
entrez:
21
6
2019
Statut:
ppublish
Résumé
Despite intense interest in human mesenchymal stromal cells (MSCs), monitoring of the progressive occurrence of senescence has been hindered by the lack of efficient detection tools. Here, the discovery of a novel MSC senescence-specific fluorescent probe (CyBC9) identified by a high-throughput screen is reported. Compared with the prototypical senescence-associated β-galactosidase (SA-β-gal) staining, the CyBC9 assay is rapid (2 h) and nontoxic and can thus be applied to live cells. It is shown that CyBC9 is able to stain early and late senescent populations both in monolayer- and in microcarrier-based cultures. Finally, to investigate the mechanism of CyBC9, colocalization assays are performed and it is found that CyBC9 is accumulated in the mitochondria of senescent MSCs presumably due to the loss of membrane potential. Taken together, it is expected that CyBC9 will be a useful tool to ameliorate cell therapy through rapid and early screening of senescent phenotypes in clinically relevant MSCs.
Identifiants
pubmed: 31218816
doi: 10.1002/biot.201800691
doi:
Substances chimiques
Fluorescent Dyes
0
Fluorescein-5-isothiocyanate
I223NX31W9
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1800691Subventions
Organisme : Joint Council Office of Biomedical Research Council (BMRC), Agency for Science, Technology and Research (A*STAR) Singapore.
Informations de copyright
© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.