Development of urea biosensor using non-covalent complexes of urease with aldehyde derivative of PEG and analysis on serum samples.
Enzyme-polymer modifications
PEG-aldehyde
non-covalent complexes
urea biosensor
urease
Journal
Preparative biochemistry & biotechnology
ISSN: 1532-2297
Titre abrégé: Prep Biochem Biotechnol
Pays: England
ID NLM: 9607037
Informations de publication
Date de publication:
2019
2019
Historique:
pubmed:
21
6
2019
medline:
18
12
2019
entrez:
21
6
2019
Statut:
ppublish
Résumé
Non-covalent complexes of urease/polyethylene glycol (PEG)-aldehyde were synthesized using regular molar ratios of urease and PEG-aldehyde at room temperature. The physical properties of the non-covalent complexes were analyzed in order to investigate the impact of coupling ratio, temperature, pH, storage stability, and thermal stability. Urease activity was analyzed by UV-Vis spectrophotometer at 630 nm. The results showed that the strongest thermal resistance was obtained using
Identifiants
pubmed: 31219372
doi: 10.1080/10826068.2019.1630650
doi:
Substances chimiques
Aldehydes
0
Enzymes, Immobilized
0
Polyethylene Glycols
3WJQ0SDW1A
Urea
8W8T17847W
Urease
EC 3.5.1.5
Types de publication
Evaluation Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM