Zika virus infection of pregnant rats and associated neurological consequences in the offspring.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2019
2019
Historique:
received:
29
12
2018
accepted:
04
06
2019
entrez:
21
6
2019
pubmed:
21
6
2019
medline:
13
2
2020
Statut:
epublish
Résumé
Zika virus (ZIKV) is a mosquito-borne flavivirus associated with microcephaly and other neurological disorders in infants born to infected mothers. Despite being declared an international emergency by the World Health Organization, very little is known about the mechanisms of ZIKV pathogenesis or the long-term consequences of maternal ZIKV infection in the affected offspring, largely due to the lack of appropriate rodent models. To address this issue, our lab has developed a working model of prenatal ZIKV infection in rats. In this study, we infected immune competent pregnant female rats with 105-107 PFU of ZIKV (PRVABC59, Puerto Rico/Human/Dec 2015) in order to examine its pathogenesis in the dams and pups. We examined the febrile response and sickness behavior in the dams, in addition to neonatal mortality, microglia morphology, cortical organization, apoptosis, and brain region-specific volumes in the offspring. Here, we demonstrate that pregnant and non-pregnant female rats have a distinct febrile response to ZIKV infection. Moreover, prenatal ZIKV infection increased cell death and reduced tissue volume in the hippocampus and cortex in the neonatal offspring. For the first time, we demonstrate the efficacy and validity of an immunocompetent rat model for maternal ZIKV infection that results in significant brain malformations in the neonatal offspring.
Identifiants
pubmed: 31220154
doi: 10.1371/journal.pone.0218539
pii: PONE-D-18-37062
pmc: PMC6586346
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0218539Subventions
Organisme : NIGMS NIH HHS
ID : P20 GM103446
Pays : United States
Organisme : NIGMS NIH HHS
ID : P20 GM103653
Pays : United States
Organisme : NICHD NIH HHS
ID : R21 HD096309
Pays : United States
Organisme : NCRR NIH HHS
ID : S10 RR027273
Pays : United States
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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