Altered expression of epigenetic regulators and imprinted genes in human placenta and fetal tissues from second trimester spontaneous pregnancy losses.
5-Methylcytosine
/ analogs & derivatives
Aborted Fetus
/ metabolism
Abortion, Spontaneous
/ genetics
Adult
Cyclin-Dependent Kinase Inhibitor p57
/ genetics
DNA Methylation
DNA-Binding Proteins
/ genetics
Dioxygenases
/ genetics
Female
Genomic Imprinting
Humans
Insulin-Like Growth Factor II
/ genetics
Placenta
/ metabolism
Pregnancy
Pregnancy Trimester, Second
Proto-Oncogene Proteins
/ genetics
RNA, Long Noncoding
/ genetics
DNA hydroxymethylation
DNA methylation
Spontaneous abortion
TET enzymes
imprinted genes
Journal
Epigenetics
ISSN: 1559-2308
Titre abrégé: Epigenetics
Pays: United States
ID NLM: 101265293
Informations de publication
Date de publication:
12 2019
12 2019
Historique:
pubmed:
22
6
2019
medline:
10
6
2020
entrez:
22
6
2019
Statut:
ppublish
Résumé
Epigenetic mechanisms such as genomic imprinting have a fundamental role in embryo and fetal development. Hence, we here studied expression levels of epigenetic modifiers and imprinted genes in cases of ididopathic spontaneous abortion (SA). Thirty-five placental samples and 35 matched fetal tissues from second trimester SA were analysed; including 16 controls (placental and fetal infections as the known cause of spontaneous abortion) and 19 idiopathic SA cases. Transcript levels of epigenetic regulators and imprinted genes were measured by qRT-PCR and methylation at imprinted genes was studied by bisulfite genomic sequencing and MS-MLPA. Global DNA hydroxymethylation (5-hmC) levels were measured by an ELISA-based assay. We observed an upregulation of
Identifiants
pubmed: 31221015
doi: 10.1080/15592294.2019.1634988
pmc: PMC6791697
doi:
Substances chimiques
CDKN1C protein, human
0
Cyclin-Dependent Kinase Inhibitor p57
0
DNA-Binding Proteins
0
H19 long non-coding RNA
0
IGF2 protein, human
0
Proto-Oncogene Proteins
0
RNA, Long Noncoding
0
5-hydroxymethylcytosine
1123-95-1
Insulin-Like Growth Factor II
67763-97-7
5-Methylcytosine
6R795CQT4H
TET3 protein, human
EC 1.-
Dioxygenases
EC 1.13.11.-
TET2 protein, human
EC 1.13.11.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1234-1244Références
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