EV-associated miRNAs from peritoneal lavage as potential diagnostic biomarkers in colorectal cancer.
Adenocarcinoma
/ diagnosis
Aged
Aged, 80 and over
Ascitic Fluid
/ metabolism
Biomarkers, Tumor
/ genetics
Case-Control Studies
Cohort Studies
Colorectal Neoplasms
/ diagnosis
Extracellular Vesicles
/ genetics
Female
Gene Expression Profiling
/ methods
Gene Expression Regulation, Neoplastic
Humans
Male
MicroRNAs
/ genetics
Middle Aged
Oligonucleotide Array Sequence Analysis
Peritoneal Lavage
Prognosis
Ascitic fluid
Biomarkers
Colon cancer
Colorectal cancer
Diagnostic
Extracellular vesicles
Liquid biopsy
Peritoneal lavage
miRNAs
Journal
Journal of translational medicine
ISSN: 1479-5876
Titre abrégé: J Transl Med
Pays: England
ID NLM: 101190741
Informations de publication
Date de publication:
20 06 2019
20 06 2019
Historique:
received:
02
04
2019
accepted:
13
06
2019
entrez:
22
6
2019
pubmed:
22
6
2019
medline:
1
7
2020
Statut:
epublish
Résumé
Colorectal cancer (CRC) is the third leading cause of cancer-related mortality worldwide. Current systematic methods for diagnosing have inherent limitations so development of a minimally-invasive diagnosis, based on the identification of sensitive biomarkers in liquid biopsies could therefore facilitate screening among population at risk. In this study, we aim to develop a novel approach to identify highly sensitive and specific biomarkers by investigating the use of extracellular vesicles (EVs) isolated from the peritoneal lavage as a source of potential miRNA diagnostic biomarkers. We isolated EVs by ultracentrifugation from 25 ascitic fluids and 25 peritoneal lavages from non-cancer and CRC patients, respectively. Analysis of the expression of EV-associated miRNAs was performed using Taqman OpenArray technology through which we could detect 371 miRNAs. 210 miRNAs were significantly dysregulated (adjusted p value < 0.05 and abs(logFC) ≥ 1). The top-10 miRNAs, which had the AUC value higher than 0.95, were miRNA-199b-5p, miRNA-150-5p, miRNA-29c-5p, miRNA-218-5p, miRNA-99a-3p, miRNA-383-5p, miRNA-199a-3p, miRNA-193a-5p, miRNA-10b-5p and miRNA-181c-5p. This finding opens the avenue to the use of EV-associated miRNA of peritoneal lavages as an untapped source of biomarkers for CRC.
Sections du résumé
BACKGROUND
Colorectal cancer (CRC) is the third leading cause of cancer-related mortality worldwide. Current systematic methods for diagnosing have inherent limitations so development of a minimally-invasive diagnosis, based on the identification of sensitive biomarkers in liquid biopsies could therefore facilitate screening among population at risk.
METHODS
In this study, we aim to develop a novel approach to identify highly sensitive and specific biomarkers by investigating the use of extracellular vesicles (EVs) isolated from the peritoneal lavage as a source of potential miRNA diagnostic biomarkers. We isolated EVs by ultracentrifugation from 25 ascitic fluids and 25 peritoneal lavages from non-cancer and CRC patients, respectively. Analysis of the expression of EV-associated miRNAs was performed using Taqman OpenArray technology through which we could detect 371 miRNAs.
RESULTS
210 miRNAs were significantly dysregulated (adjusted p value < 0.05 and abs(logFC) ≥ 1). The top-10 miRNAs, which had the AUC value higher than 0.95, were miRNA-199b-5p, miRNA-150-5p, miRNA-29c-5p, miRNA-218-5p, miRNA-99a-3p, miRNA-383-5p, miRNA-199a-3p, miRNA-193a-5p, miRNA-10b-5p and miRNA-181c-5p.
CONCLUSIONS
This finding opens the avenue to the use of EV-associated miRNA of peritoneal lavages as an untapped source of biomarkers for CRC.
Identifiants
pubmed: 31221189
doi: 10.1186/s12967-019-1954-8
pii: 10.1186/s12967-019-1954-8
pmc: PMC6585099
doi:
Substances chimiques
Biomarkers, Tumor
0
MicroRNAs
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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